Time-Dependent Protective Efficacy of Trolox (Vitamin E Analog) Against Microcystin-Induced Toxicity in Tilapia (Oreochromis niloticus)

被引:38
作者
Prieto, Ana Isabel [1 ]
Jos, Angeles [1 ]
Pichardo, Silvia [1 ]
Moreno, Isabel [1 ]
Alvarez de Sotomayor, Maria [2 ]
Moyano, Rosario [3 ]
Blanco, Alfonso [3 ]
Camean, Ana Maria [1 ]
机构
[1] Univ Seville, Fac Pharm, Area Toxicol, Seville, Spain
[2] Univ Seville, Fac Pharm, Dept Pharmacol, Seville, Spain
[3] Univ Cordoba, Dept Anat & Comparat Pathol & Anat, E-14071 Cordoba, Spain
关键词
cyanobacteria; microcystin; tilapia; oxidative stress; vitamin E; Trolox; OXIDATIVE STRESS RESPONSES; ANTIOXIDANT DEFENSE-MECHANISMS; GLUTATHIONE S-TRANSFERASES; DIFFERENT TROPHIC LEVELS; FRESH-WATER FISH; LABORATORY CONDITIONS; LIPID-PEROXIDATION; IN-VIVO; HEPATOTOXIC MICROCYSTINS; CYANOBACTERIAL BLOOMS;
D O I
10.1002/tox.20458
中图分类号
X [环境科学、安全科学];
学科分类号
08 ; 0830 ;
摘要
Microcystins (MCs), hepatotoxins from cyanobacteria, induce oxidative stress and pathological changes in fish that can be ameliorated with chemoprotectants such as vitamin E (vit E). This study investigated the time period after MCs exposure in which Trolox, a vitamin E analog, is effective against oxidative and histological damage in different organs of tilapia (Oreochromis niloticus). Fish were fed Trolox supplement (700 mg/kg diet) for 7 days, or received only commercial fish food, and then were exposed to a single oral dose of 120 mu g/fish microcystin-LR, and sacrificed in 24, 48, or 72 h. The Trolox protective efficacy was evaluated based on lipid peroxidation (LPO), protein oxidation, enzymatic and non-enzymatic antioxidants, and a morphologic study. Regarding the oxidative stress biomarkers altered by MCs, the higher protective action of Trolox was observed 24 h post toxin exposure, although it extends also until 48 h in gills (superoxide dismutase (SOD), catalase (CAT)), and liver, where glutathione reductase (GR) backed to control values 48 and 72 h after the toxin application. Glutathione-S-transferase (GST) activity in the liver was ameliorated by the chemoprotectant after 24 and 48 h, although control values were not recovered. Trolox modulation of these biomarkers and its ability to quench free radicals explain the recovery of LPO values in all organs at 24 h and also in gills at 48 h. Histopathologically, Trolox efficacy was more evident after 72 h. (C) 2008 Wiley Periodicals, Inc. Environ Toxicol 54: 563-579, 2009.
引用
收藏
页码:563 / 579
页数:17
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