Formation of a 2-methyl-branched fatty aldehyde during peroxisomal alpha-oxidation

被引:43
作者
Croes, K [1 ]
Casteels, M [1 ]
Asselberghs, S [1 ]
Herdewijn, P [1 ]
Mannaerts, GP [1 ]
VanVeldhoven, PP [1 ]
机构
[1] REGA INST,B-3000 LOUVAIN,BELGIUM
关键词
aldehyde; alpha-oxidation; formate; peroxisome; phytanic acid; Refsum's disease;
D O I
10.1016/S0014-5793(97)00856-9
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
In the final reaction of peroxisomal alpha-oxidation of 3-methyl-branched fatty acids a 2-hydroxy-3-methylacyl-CoA intermediate is cleaved to formyl-CoA and a hitherto unidentified product, The release of formyl-CoA suggests that the unidentified product may be a fatty aldehyde. When purified rat liver peroxisomes were incubated with 2-hydroxy-3-methylhexadecanoyl-CoA 2-methylpentadecanal was indeed formed, The production rates of formyl-CoA (measured as formate) and of the aldehyde were in the same range, While the production of formate remained unaltered in the presence of NAD(+), the amount of 2-methylpentadecanal was decreased, which was accompanied by the formation of 2-methylpentadecanoic acid. These data indicate that (1) during alpha-oxidation the 2-hydroxy-3-methylacyl-CoA is cleaved to a 2-methyl-branched aldehyde and formyl-CoA and (2) liver peroxisomes are capable of converting this aldehyde to a 2-methyl-branched fatty acid. (C) 1997 Federation of European Biochemical Societies.
引用
收藏
页码:643 / 645
页数:3
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