Expression of a boiling-stable protein (BsCyp) in response to heat shock, drought and ABA treatments in Sorghum bicolor

被引：5
|
作者：
Sharma, Arun Dev [1 ]
Vasudeva, Ravia [1 ]
Kaur, Ramandeep [1 ]
机构：
[1] Lyallpur Khalsa Coll, Dept Biotechnol, Jalandhar 144001, Punjab, India
来源：
PLANT GROWTH REGULATION | 2006年 / 50卷 / 2-3期
关键词：
boiling-stable proteins; cyclophilin; water stress; sorghum;
D O I：
10.1007/s10725-006-9134-2
中图分类号：
Q94 [植物学];
学科分类号：
071001 ;
摘要：
The effect of heat stress, drought and abscisic acid (ABA) on the induction of boiling-stable proteins were studied in 12 h germinated sorghum embryos and endosperm. SDS-PAGE revealed the heat shock induction of one major 97 kDa boiling-stable protein both in embryos and endosperm. Western blotting analysis, using polyclonal antibodies raised against a 20 kDa Arabidopsis thaliana cyclophilin, revealed the induction of a cross-reacting band (97 kDa, BsCyp) in both embryos and endosperm in a time dependent manner. The BsCyp boiling-stable polypeptide was also regulated by exogenous application of ABA, indicating its role in water stress adaptation. Drought stress also resulted in a significant induction of boiling-stable protein (BsCyp) in both embryos and endosperm. Based upon these observations the possible role of BsCyp in water stress tolerance is discussed.
引用
收藏
页码：249 / 254
页数：6
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