Fluorescence Spectroscopy Study of Single-Stranded Nucleic Acid Aptamer Species Against NS5 Zika Virus

被引：1

作者：

Abalo, A. A. ^{[1
]}

Argondizzo, A. P. C. ^{[2
]}

Morais, L. M. ^{[3
]}

Alves, L. N. ^{[4
]}

Silva, D. ^{[1
]}

Valdez, E. C. N. ^{[5
]}

Missailidis, S. ^{[6
]}

机构：

[1] BioManguinhos Fiocruz, Pavilhao Rockfeller, Lab Tecnol Anticorpos Monoclonais, Av Brasil 4365, BR-21040900 Rio De Janeiro, RJ, Brazil

[2] BioManguinhos Fiocruz, Pavilhao Rockfeller, Lab Tecnol Recombinante, Av Brasil 4365, BR-21040900 Rio De Janeiro, RJ, Brazil

[3] BioManguinhos Fiocruz, Lab Tecnol Virol, Edif Rocha Lima,Av Brasil 4365, BR-21040900 Rio De Janeiro, RJ, Brazil

[4] Int Ctr Genet Engn & Biotechnol, ICGEB, Localita Padriciano 99, I-34149 Trieste, TS, Italy

[5] Univ Estado Rio De Janeiro, Programa Posgrad Ciencias Med, R Sao Francisco Xavier 524, BR-20550900 Rio De Janeiro, RJ, Brazil

[6] BioManguinhos Fiocruz, Desenvolvimento Tecnol, Edif Rocha Lima,Av Brasil 4365, BR-21040900 Rio De Janeiro, RJ, Brazil

来源：

PROCEEDINGS OF THE INTERNATIONAL CONFERENCE OF COMPUTATIONAL METHODS IN SCIENCES AND ENGINEERING 2019 (ICCMSE-2019) | 2019年 / 2186卷

关键词：

D O I：

10.1063/1.5138052

中图分类号：

O29 [应用数学];

学科分类号：

070104 ;

摘要：

Zika virus (ZIKV) belongs to the Flaviviridae family and its recent outbreak and association with human diseases (e.g. neurological disorders) have raised global health concerns. The viral NS5 protein is the most conserved ZIKV protein, and is comprised of two domains, an N-terminal methyltransferase domain and an RNA-dependent RNA polymerase (RdRP) domain at the C-terminal end. The fact that viral NS5 is essential for viral replication but lacks a human counterpart makes NS5 an important target to be studied. Single-stranded DNA oligonucleotides aptamers can fold into unique three-dimensional conformations that recognize targets with high-specific and affinity. This work aims to study single-stranded DNA aptamers displaying high -affinity and specificity generated against ZIKV NS5 protein to be used in diagnostic platforms.

引用

页数：4

共 14 条

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