Five novel bifidobacterial species isolated from faeces of primates in two Czech zoos: Bifidobacterium erythrocebi sp. nov., Bifidobacterium moraviense sp. nov., Bifidobacterium oedipodis sp. nov., Bifidobacterium olomucense sp. nov. and Bifidobacterium panos sp. nov.

被引：10

作者：

Neuzil-Bunesova, Vera ^{[1
]}

Lugli, Gabriele Andrea ^{[2
]}

Modrackova, Nikol ^{[1
]}

Vlkova, Eva ^{[1
]}

Bolechova, Petra ^{[3
]}

Burtscher, Johanna ^{[4
]}

Longhi, Giulia ^{[2
]}

Mancabelli, Leonardo ^{[2
]}

Killer, Jiri ^{[1
,5
]}

Domig, Konrad ^{[4
]}

Ventura, Marco ^{[2
]}

机构：

[1] Czech Univ Life Sci Prague, Dept Microbiol Nutr & Dietet, Kamycka 129, Prague 16500 6, Czech Republic

[2] Univ Parma, Dept Chem Life Sci & Environm Sustainabil, Lab Probiogenom, Parma, Italy

[3] Czech Univ Life Sci Prague, Dept Ethol & Compan Anim Sci, Kamycka 129, Prague 16500 6, Czech Republic

[4] Univ Nat Resources & Life Sci Vienna BOKU, Inst Food Sci, Dept Food Sci & Technol, Muthgasse 18, A-1190 Vienna, Austria

[5] Czech Acad Sci, Inst Anim Physiol & Genet, Vvi, Videnska 1083, Prague 14220 4, Czech Republic

来源：

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY | 2021年 / 71卷 / 01期

关键词：

Bifidobacterium; captivity; FAMEs; monkeys; phylogenomics; taxonomy;

D O I：

10.1099/ijsem.0.004573

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Five Bifidobacterium strains, VB23(T), VB24(T), VB25(T), VB26(T) and VB31(T), were isolated from chimpanzee (Pan troglodytes), cotton-top tamarin (Saguinus oedipus), Goeldi's marmoset (Callimico goeldii), moustached tamarin (Saguinus mystax) and patas monkey (Erythrocebus patas), respectively, which were kept in two Czech zoos. These strains were isolated from faecal samples and were Gram-positive, non-motile, non-sporulating, anaerobic and fructose-6-phosphate phosphoketolase-positive. Phylogenetic analyses based on 16S rRNA revealed close relatedness between VB23(T) and Bifidobacterium angulatum LMG 11039(T) (96.0%), VB24(T) and Bifidobacterium pullorum subsp. pullorum DSM 20433(T) (96.1%), VB25(T) and Bifidobacterium goeldii LMG 30939(T) (96.5%), VB26(T) and Bifidobacterium imperatoris LMG 30297(T) (98.1%), and VB31(T) and B. angulatum LMG 11039(T) (99.40 %). Internal transcribed spacer profiling revealed that VB23(T), VB24(T), VB25(T), VB26(T) and VB31(T) had highest similarity to Bifidobacterium breve LMG 13208(T) (77.2%), Bifidobacterium longum subsp. infantis ATCC 15697(T) (85.8 %), Bifidobacterium biavatii DSM 23969(T) (76.9%), B. breve LMG 13208(T) (81.2%) and B. angulatum LMG 11039(T) (88.2 %), respectively. Average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) analyses with their closest neighbours supported the independent phylogenetic positions of the strains with values between 86.3 and 94.3 % for ANI and 25.8 and 54.9 % for dDDH. These genomic and phylogenetic analyses suggested that the evaluated strains were novel Bifidobacterium species named Bifidobacterium erythrocebi sp. nov. (VB31(T)=DSM 109960(T)=CCUG 73843(T)), Bifidobacterium moraviense sp. nov. (VB25(T)=DSM 109958(T)=CCUG 73842(T)), Bifidobacterium oedipodis sp. nov. (VB24(T)=DSM 109957(T)=CCUG 73932(T)), Bifidobacterium olomucense sp. nov. (VB26(T)=DSM 109959(T)=CCUG 73845(T)) and Bifidobacterium panos sp. nov. (VB23(T)=DSM 109963(T)=CCUG 73840(T)).

引用

页码：1 / 12

页数：12

共 42 条

[1] SPAdes: A New Genome Assembly Algorithm and Its Applications to Single-Cell Sequencing [J].