Concentration-dependent behavior of nisin interaction with supported bilayer lipid membrane

被引：27
作者：
Huang, WM [1 ]
Zhang, ZL [1 ]
Han, XJ [1 ]
Wang, JG [1 ]
Tang, JL [1 ]
Dong, SJ [1 ]
Wang, EK [1 ]
机构：
[1] Chinese Acad Sci, Changchun Inst Appl Chem, State Key Lab Electroanalyt Chem, Changchun 130022, Jilin, Peoples R China
来源：
BIOPHYSICAL CHEMISTRY | 2002年 / 99卷 / 03期
基金：
中国国家自然科学基金;
关键词：
nisin; supported bilayer lipid membrane; a.c; impedance; cyclic votammetry; marker ions;
D O I：
10.1016/S0301-4622(02)00227-2
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Nisin is a positively charged antibacterial peptide that binds to the negatively charged membranes of gram-positive bacteria. The initial interaction of the peptide with the model membrane of negatively charged DPPG (dipalmitoylphosphatidylglycerol) was studied by cyclic voltammetry and a.c. impedance spectroscopy. Nisin could induce pores the supported bilayer lipid membrane, thus, it led to the marker ions Fe(CN)(6)(3-/4-) crossing the lipid membrane and giving the redox reaction on the glassy carbon electrode (GCE). Experimental results suggested that the pore formation on supported bilayer lipid membrane was dependent on the concentration of nisin and it included three main concentration stages: low, middling, high concentration. (C) 2002 Elsevier Science B.V. All rights reserved.
引用
收藏
页码：271 / 279
页数：9
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