Obestatin/ghrelin cells in normal mucosa and endocrine tumours of the stomach

被引:23
作者
Tsolakis, Apostolos V. [1 ]
Grimelius, Lars [3 ]
Stridsberg, Mats [2 ]
Falkmer, Sture E. [4 ,5 ]
Waldum, Helge L. [6 ]
Saras, Jan [1 ]
Janson, Eva T. [1 ]
机构
[1] Univ Hosp, Dept Med Sci, Sect Endocrine Oncol, S-75185 Uppsala, Sweden
[2] Univ Hosp, Dept Clin Chem, S-75185 Uppsala, Sweden
[3] Univ Hosp, Dept Genet & Pathol, S-75185 Uppsala, Sweden
[4] St Olavs Univ Hosp, Dept Pathol, N-7006 Trondheim, Norway
[5] Ryhov Cty Hosp, Dept Clin Pathol & Cytol, S-55185 Jonkoping, Sweden
[6] St Olavs Univ Hosp, Dept Gastroenterol, N-7006 Trondheim, Norway
关键词
VESICULAR MONOAMINE TRANSPORTER; IMMUNOREACTIVE CELLS; GHRELIN EXPRESSION; ACYLATED PEPTIDE; FOOD-INTAKE; HUMANS; PLASMA; GASTROPARESIS; RECEPTOR; TISSUES;
D O I
10.1530/EJE-09-0001
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Objective: Obestatin and ghrelin are derived from the same gene and co-expressed in the same endocrine cells. Vesicular monoanline transporter-2 (VMAT-2). a marker for enterochromaffin-like (ECL) cells, is considered to be expressed in ghrelin cells. The aim was to establish if the two peptides and the transporter are co-expressed, both in normal gastric rnucosa and in gastric endocrine tumours. Design: An immunohistochemical study was performed oil gastric biopsy material and on Surgical specimens from 63 patients with gastric endocrine tumours and from individuals with normal gastric mucosa. Cells displaying obestatin immunoreactivity were examined regarding co-localization with ghrelin and VMAT-2. Both single- and double-immunostaining techniques were applied. Obestatin concentration in blood was measured in a subgroup of these patients. The results were correlated to various clinico-pathological parameters. Results: In the normal mucosa, obestatin/ghrelin-immunoreactive cells rarely co-expressed VMAT-2. In most tumour tissue specimens, only a fraction of neoplastic cells displayed immunoreactivity to obestatin, and these cells always co-expressed ghrelin. Neoplastic obestatin-/ghrelin-IR cells invariably expressed VMAT-2, except for two ghrelinomas. The obestatin concentrations in blood were consistently low and did not correlate to clinico-pathological data. Conclusions: Obestatin and ghrelin immnoreactivity always occurred in the same endocrine cells ill the gastric mucosa but these Cells Only occasionally co-expressed VMAT-2, opposite to the findings in tumours. These results indicate that endocrine cells expressing obestatin and ghrelin mainly differ from VMAT-2 expressing cells (ECL-cells) and can develop into pure ghrelinomas. Plasma concentrations of obestatin did not correlate to cellular expression.
引用
收藏
页码:941 / 949
页数:9
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