Evolution of Amber Suppressor tRNAs for Efficient Bacterial Production of Proteins Containing Nonnatural Amino Acids

被引：141

作者：

Guo, Jiantao ^{[1
,2
]}

Melancon, Charles E., III ^{[1
,2
]}

Lee, Hyun Soo ^{[1
,2
]}

Groff, Dan ^{[1
,2
]}

Schultz, Peter G. ^{[1
,2
]}

机构：

[1] Scripps Res Inst, Dept Chem, La Jolla, CA 92037 USA

[2] Scripps Res Inst, Skaggs Inst Chem Biol, La Jolla, CA 92037 USA

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2009年 / 48卷 / 48期

关键词：

amber suppression; evolution; nonnatural amino acids; protein modifications; tRNA; ELONGATION-FACTOR TU; ESCHERICHIA-COLI; GENETIC-CODE; SPECIFICITY; RECOGNITION; SYNTHETASE; BINDING; SITE; BIOSYNTHESIS; CODONS;

D O I：

10.1002/anie.200904035

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Applied evolution: Regions of the M.jannaschii tyrosyl tRNACUA thought to interact with elongation factor Tu were randomized, and the resulting tRNA libraries were subjected to in vitro evolution. The tRNAs identified resulted in significantly improved yields of proteins containing nonnatural amino acids. In some cases, the degree of improvement varied in an amino acid dependent manner. © 2009 Wiley-VCH Verlag GmbH & Co. KGaA.

引用

页码：9148 / 9151

页数：4

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