Nanowire Based Guidance of the Morphology and Cytotoxic Activity of Natural Killer Cells

被引：16

作者：

Bhingardive, Viraj ^{[1
,2
]}

Le Saux, Guillaume ^{[1
,2
]}

Edri, Avishay ^{[3
]}

Porgador, Angel ^{[3
]}

Schvartzman, Mark ^{[1
,2
]}

机构：

[1] Ben Gurion Univ Negev, Dept Mat Engn, POB 653, IL-84105 Beer Sheva, Israel

[2] Ben Gurion Univ Negev, Ilse Katz Inst Nanoscale Sci & Technol, Shraga Segal Dept Microbiol, POB 653, IL-84105 Beer Sheva, Israel

[3] Ben Gurion Univ Negev, Genet Fac Hlth Sci, POB 653, IL-84105 Beer Sheva, Israel

来源：

SMALL | 2021年 / 17卷 / 14期

基金：

以色列科学基金会;

关键词：

biofunctionalization; immunotherapy; natural killer cells; silicon nanowires; site selective functionalization; IMMUNOLOGICAL SYNAPSE; T-CELLS; ACTIVATION; RECEPTOR; GROWTH; COMMUNICATION; CD107A;

D O I：

10.1002/smll.202007347

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

The cytotoxic activity of natural killer (NK) cells is regulated by many chemical and physical cues, whose integration mechanism is still obscure. Here, a multifunctional platform is engineered for NK cell stimulation, to study the effect of the signal integration and spatial heterogeneity on NK cell function. The platform is based on nanowires, whose mechanical compliance and site-selective tip functionalization with antigens produce the physical and chemical stimuli, respectively. The nanowires are confined to micron-sized islands, which induce a splitting of the NK cells into two subpopulations with distinct morphologies and immune responses: NK cells atop the nanowire islands display symmetrical spreading and enhanced activation, whereas cells lying in the straits between the islands develop elongated profiles and show lower activation levels. The demonstrated tunability of NK cell cytotoxicity provides an important insight into the mechanism of their immune function and introduces a novel technological route for the ex vivo shaping of cytotoxic lymphocytes in immunotherapy.

引用

页数：12

共 75 条

[1]

Abuillan W., 2013, DISS

[2] Relationship between CD107a expression and cytotoxic activity [J].