RainbowSTORM: an open-source ImageJ plug-in for spectroscopic single-molecule localization microscopy (sSMLM) data analysis and image reconstruction

被引：10

作者：

Davis, Janel L. ^{[1
]}

Soetikno, Brian ^{[1
]}

Song, Ki-Hee ^{[1
]}

Zhang, Yang ^{[1
]}

Sun, Cheng ^{[2
]}

Zhang, Hao F. ^{[1
]}

机构：

[1] Northwestern Univ, Dept Biomed Engn, Evanston, IL 60208 USA

[2] Northwestern Univ, Dept Mech Engn, Evanston, IL 60208 USA

来源：

BIOINFORMATICS | 2020年 / 36卷 / 19期

基金：

美国国家科学基金会;

关键词：

D O I：

10.1093/bioinformatics/btaa635

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A Summary: Spectroscopic single-molecule localization microscopy (sSMLM) simultaneously captures the spatial locations and full spectra of stochastically emitting fluorescent single molecules. It provides an optical platform to develop new multimolecular and functional imaging capabilities. While several open-source software suites provide subdiffraction localization of fluorescent molecules, software suites for spectroscopic analysis of sSMLM data remain unavailable. RainbowSTORM is an open-source ImageJ/FIJI plug-in for end-to-end spectroscopic analysis and visualization for sSMLM images. RainbowSTORM allows users to calibrate, preview and quantitatively analyze emission spectra acquired using different reported sSMLM system designs and fluorescent labels.

引用

页码：4972 / 4974

页数：3

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