Trichothecenes NIV and DON modulate the maturation of murine dendritic cells

被引：26
作者：
Luongo, D. [1 ,2 ]
Severino, L. [2 ]
Bergamo, P. [1 ]
D'Arienzo, R. [1 ]
Rossi, M. [1 ]
机构：
[1] CNR, Ist Sci Alimentaz, Inst Food Sci, I-83100 Avellino, Italy
[2] Univ Naples Federico II, Dept Pathol & Anim Hlth, I-80137 Naples, Italy
来源：
TOXICON | 2010年 / 55卷 / 01期
关键词：
Mycotoxins; Dendritic cells; Immunotoxicity; Cytokines; ACTIVATED PROTEIN-KINASE; NITRIC-OXIDE SYNTHASE; IN-VITRO EXPOSURE; T-CELLS; FUSARIUM MYCOTOXINS; VOMITOXIN DEOXYNIVALENOL; CYTOKINE PRODUCTION; MACROPHAGE MODEL; INTERFERON-GAMMA; TNF-ALPHA;
D O I：
10.1016/j.toxicon.2009.06.039
中图分类号：
R9 [药学];
学科分类号：
1007 ;
摘要：
Nivalenol (NIV) and Deoxynivalenol (DON), mycotoxins of the trichothecene family are considered very common food contaminants. In this work, we investigated whether the immunotoxic effects ascribed to these trichothecenes may be mediated by perturbations in the activity of dendritic cells (DCs). Murine bone marrow-derived DCs were used to evaluate the effects of NIV and DON on the LPS-induced maturation process. We found that the expression of the class 11 MHC and of the accessory CD11c molecules, but not of the costimulatory CD86 marker, was down-regulated by NIV and DON exposure in LPS-treated DCs, as well as nitric oxide (NO) production. Interestingly, NIV, but not DON, induced DC necrosis. Moreover, the analysis of the cytokine pattern showed that IL-12 and IL-10 expressions induced by LPS exposure were suppressed by both trichothecenes in a dose-dependent fashion. on the other hand, the secretion of the proinflammatory cytokine TNF-alpha was increased as a direct consequence of DON and NIV exposure. Taken together, our data indicated that the immunotoxicity of NIV and DON was related to the capacity of both trichothecenes to interfere with phenotypic and functional features of maturing DCs. (C) 2009 Elsevier Ltd. All rights reserved.
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收藏
页码：73 / 80
页数：8
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