Expression of Hepatitis B Surface Antigen Gene in Ginseng Cells

被引：0

作者：

Yu Hai-peng ^{[2
]}

Xue Yan ^{[2
]}

An Wei ^{[4
]}

Liu Dan ^{[1
]}

Hao Shu-mei ^{[3
]}

Sheng Jun ^{[1
]}

机构：

[1] Changchun Inst Biol Prod, Changchun 130062, Peoples R China

[2] Jilin Univ, Coll Life Sci, Changchun 130012, Peoples R China

[3] Changchun Univ Sci & Technol, Changchun 130022, Peoples R China

[4] Jilin Univ, Dept Organ Transplantat, Hosp 1, Changchun 130021, Peoples R China

来源：

CHEMICAL RESEARCH IN CHINESE UNIVERSITIES | 2009年 / 25卷 / 05期

关键词：

Transgenic cell; HBsAg; Ginseng; TRANSGENIC PLANTS; ORAL IMMUNIZATION; EDIBLE VACCINE; IMMUNOGENICITY; TRANSFORMATION; TUMEFACIENS; PROMOTER; CULTURE; TOBACCO; VIRUS;

D O I：

暂无

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

The recombinant plasmid pBIBSa containing the HBsAg DNA fragment was transferred into Agrobacterium tumefaciens strain LBA4404 directly. Ginseng cells were transfected with A. tumefaciens carrying pBIBSa and the ginseng cell lines carrying HBsAg-S gene were obtained. The presence of target gene in the transfect cells was confirmed by PCR and RT-PCR. A clear band at the site of 700 bp was observed by agarose electrophoresis analysis of the samples containing the target gene. HBsAg expressed by the transgenic ginseng cells was detected by Western blot. Maximum expression levels of 184 ng HBsAg/g FW and 0.009% of the total soluble proteins were observed by ELISA. HBsAg in ginseng cells was located both on the cell membrane and in the nuclei.

引用

页码：695 / 698

页数：4

共 22 条

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