This scientific project is conducted to identify and determine the comfort level in the lecturer room, Center of Technology in Marine Engineering (CTME), Politeknik Ungku Omar. Air quality control and comfortable room are important to ensure that occupants feel at ease during everyday activities especially in the lecturer's room. Comfortable conditions will contribute towards the effectiveness of dailywork processes. This level of comfort refers to the ISO 7730 Standard. The parameters measured and used in the calculation on this study are air temperature, mean radial temperature, relative humidity and Carbon Dioxide (CO2) level in the Lecture room. Measurements were carried out using the Thermal Comfort Multistation (TCM). The data obtained were analyzed descriptively through the plotting of the relevant graphs. This study also presents the importance of temperature and content of Carbon dioxide (CO2) around the human comfort especially in indoor space. This factor encourages theworker to be in a safe, comfortable environment and thereby enhances job satisfaction and performance.