Viral etiology of acute respiratory infections in children in Southern Iran

被引:13
作者
Halaji, Mehrdad [1 ]
Hashempour, Tayebeh [1 ]
Moayedi, Javad [1 ]
Pouladfar, Gholam Reza [1 ]
Khansarinejad, Behzad [2 ]
Khashei, Reza [3 ]
Moattari, Afagh [3 ]
Musavi, Zahra [1 ]
Ghassabi, Farzaneh [1 ]
Pirbonyeh, Neda [3 ]
机构
[1] Shiraz Univ Med Sci, Nemazee Teaching Hosp, Clin Microbiol Res Ctr, Shiraz, Iran
[2] Arak Univ Med Sci, Dept Microbiol & Immunol, Arak, Iran
[3] Shiraz Univ Med Sci, Sch Med, Dept Bacteriol & Virol, Shiraz, Iran
关键词
Acute respiratory infections; Respiratory syncytial virus; Influenza A virus; Human metapneumovirus; Real-time polymerase chain reaction; SYNCYTIAL VIRUS; HUMAN METAPNEUMOVIRUS; TRACT INFECTIONS;
D O I
10.1590/0037-8682-0249-2018
中图分类号
R38 [医学寄生虫学]; Q [生物科学];
学科分类号
07 ; 0710 ; 09 ; 100103 ;
摘要
Introduction: Prevalence of influenza A virus (Flu-A), respiratory syncytial virus (RSV), and human metapneumovirus (hMPV) w as assessed in children with acute respiratory infections (ARIs). Methods: Nasopharyngeal aspirates and throat swabs were subjected to real-time polymerase chain reaction (PCR) to detect RSV and Flu-A and to conventional PCR to detect hMPV. Results: Of the 156 children assessed, 93 (59.6%) carried at least one virus, with 35.9% positive for RSV. 14.1% for hMPV, and 9.6% for Flu-A. The prevalence of co-infections was 2.6%. Conclusions: The high detection rate may reflect increased sensitivity of real-time PCR compared to traditional PCR and viral culture.
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页数:4
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