Overproduction of the Pro-Apoptotic Molecule, Programmed Cell Death 5, in Toxoplasma gondii Leads to Increased Apoptosis of Host Macrophages

被引：7

作者：

Bannai, Hiroshi ^{[1
]}

Nishikawa, Yoshifumi ^{[1
]}

Ibrahim, Hany Mohamed ^{[1
]}

Yamada, Kyoko ^{[1
]}

Kawase, Osamu ^{[1
]}

Watanabe, Jun-ichi ^{[2
]}

Sugimoto, Chihiro ^{[3
]}

Xuan, Xuenan ^{[1
]}

机构：

[1] Obihiro Univ Agr & Vet Med, Natl Res Ctr Protozoan Dis, Obihiro, Hokkaido 0808555, Japan

[2] Univ Tokyo, Inst Med Sci, Minato Ku, Tokyo 1088639, Japan

[3] Hokkaido Univ, Res Ctr Zoonosis Control, Chuou Ku, Sapporo, Hokkaido 0600818, Japan

来源：

JOURNAL OF VETERINARY MEDICAL SCIENCE | 2009年 / 71卷 / 09期

基金：

日本学术振兴会;

关键词：

apoptosis; overexpression; PDCD5; Toxoplasma gondii; HUMAN IMMUNODEFICIENCY VIRUS; T-CELLS; DEPENDENT APOPTOSIS; INFECTION; PROTEIN; GAMMA; MICE; OVEREXPRESSION; MECHANISMS; ANTIBODY;

D O I：

10.1292/jvms.71.1183

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

We established a recombinant strain of Toxoplasma gondii that overexpressed programmed cell death 5 (TgPDCD5), in order to evaluate the role of endogenous TgPDCD5 in macrophage apoptosis during T gondii infection. Immunofluorescence microscopy revealed that overproduced TgPDCD5 with a hemagglutinin tag was localized in the cytosol, which was consistent with the localization of endogenous TgPDCD5. The induced TgPDCD5-HA was recognized as ail additional band by Western blot analysis, indicating successful overexpression of TgPDCD5. Secretion and release of TgPDCD5 by the parasite was also up-regulated in a time-dependent manner. which reflected its overproduction. Apoptosis due to parasite infection and interferon-gamma treatment was significantly upregulated by the overexpression of TgPDCD5. These results Suggest that endogenous TgPDCD5 plays a role in macrophage apoptosis during T. gondii infection.

引用

页码：1183 / 1189

页数：7

共 39 条

[1] Programmed Cell Death 5 from Toxoplasma gondii:: A secreted molecule that exerts a pro-apoptotic effect on host cells [J].

Bannai, Hiroshi ;

Nishikawa, Yoshifumi ;

Matsuo, Tomohide ;

Kawase, Osamu ;

Watanabe, Junichi ;

Sugimoto, Chihiro ;

Xuan, Xuenan .

MOLECULAR AND BIOCHEMICAL PARASITOLOGY, 2008, 159 (02) :112-120

[2] Nuclear translocation of PDCD5 (TFAR19): an early signal for apoptosis? [J].

Chen, YY ;

Sun, RH ;

Han, WL ;

Zhang, YM ;

Song, QS ;

Di, CH ;

Ma, DL .

FEBS LETTERS, 2001, 509 (02) :191-196

[3]

Dubey J.P., 1988, TOXOPLASMOSIS ANIMAL

[4] Intercellular trafficking and protein delivery by a herpesvirus structural protein [J].

Elliott, G ;

OHare, P .

CELL, 1997, 88 (02) :223-233

[5] CELLULAR UPTAKE OF THE TAT PROTEIN FROM HUMAN IMMUNODEFICIENCY VIRUS [J].

FRANKEL, AD ;

PABO, CO .

CELL, 1988, 55 (06) :1189-1193

[6] Interleukin-12 p40-and Fas ligand-dependent apoptotic pathways involving STAT-1 phosphorylation are triggered during infection with a virulent strain of Toxoplasma gondii [J].

Gavrilescu, LC ;

Denkers, EY .

INFECTION AND IMMUNITY, 2003, 71 (05) :2577-2583

[7] IFN-γ overproduction and high level apoptosis are associated with high but not low virulence Toxoplasma gondii infection [J].

Gavrilescu, LC ;

Denkers, EY .

JOURNAL OF IMMUNOLOGY, 2001, 167 (02) :902-909

[8] AUTONOMOUS FUNCTIONAL DOMAINS OF CHEMICALLY SYNTHESIZED HUMAN IMMUNODEFICIENCY VIRUS TAT TRANS-ACTIVATOR PROTEIN [J].

GREEN, M ;

LOEWENSTEIN, PM .

CELL, 1988, 55 (06) :1179-1188

[9] Overexpression, purification and localization of apoptosis related protein from Plasmodium falciparum [J].

Guha, Mithu ;

Choubey, Vinay ;

Maity, Pallab ;

Kumar, Sanjay ;

Shrivastava, Kunikum ;

Puri, Sunil K. ;

Bandyopadhyay, Uday .

PROTEIN EXPRESSION AND PURIFICATION, 2007, 52 (02) :363-372

[10] Inhibition of apoptosis by intracellular protozoan parasites [J].

Heussler, VT ;

Küenzi, P ;

Rottenberg, S .

INTERNATIONAL JOURNAL FOR PARASITOLOGY, 2001, 31 (11) :1166-1176

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