DHEA-Bodipy-A functional fluorescent DHEA analog for live cell imaging

被引：14
作者：
Lemcke, Susanne [1 ]
Hoennscheidt, Christoph [1 ]
Waschatko, Gustav [1 ]
Bopp, Anita [1 ]
Luetjohann, Dieter [2 ]
Bertram, Norbert [3 ]
Gehrig-Burger, Katja [1 ]
机构：
[1] Johannes Gutenberg Univ Mainz, Inst Biochem, D-55099 Mainz, Germany
[2] Univ Clin Bonn, Ctr Internal Med, Lab Special Lipid Diagnost, Inst Clin Chem & Pharmacol, D-53127 Bonn, Germany
[3] Labor Toxikol & Analyt, D-53639 Konigswinter, Germany
来源：
MOLECULAR AND CELLULAR ENDOCRINOLOGY | 2010年 / 314卷 / 01期
关键词：
DHEA-Bodipy; Genomic; Non-genomic; Fluorescence microscopy; Live cell imaging; Trafficking; DEHYDROEPIANDROSTERONE-SULFATE; METABOLISM; RECEPTORS; ANDROGENS; STIMULATE;
D O I：
10.1016/j.mce.2009.09.006
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
The androgen dehydroepiandrosterone (DHEA) has been reported to protect neuronal cells against dysfunction and apoptosis. Several signaling pathways involved in these effects have been described but little is known about the intracellular trafficking of DHEA. We describe design, synthesis and characterization of DHEA-Bodipy, a novel fluorescent DHEA analog. DHEA-Bodipy proved to be a functional DHEA derivative: DHEA-Bodipy (i) induced estrogen receptor alpha-mediated gene activation, (ii) protected PC12 rat pheochromocytoma cells against serum-deprivation-induced apoptosis, and (iii) induced stress fibers and focal adhesion contacts in SH-SY5Y human neuroblastoma cells. DHEA-Bodipy bound rapidly and specifically to plasma membranes of living PC12 cells. We analyzed metabolism and trafficking of DHEA-Bodipy in human neuroblastoma cells. DHEA-Bodipy is the first functional fluorescent DHEA derivative suitable for live cell imaging of intracellular DHEA transport and localization. (C) 2009 Elsevier Ireland Ltd. All rights reserved.
引用
收藏
页码：31 / 40
页数：10
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