Characterization of microsatellite loci in sea urchins (Strongylocentrotus spp.)

被引:13
作者
Addison, JA [1 ]
Hart, MW [1 ]
机构
[1] Dalhousie Univ, Dept Biol, Halifax, NS B3H 4J1, Canada
来源
MOLECULAR ECOLOGY NOTES | 2002年 / 2卷 / 04期
关键词
echinoderm; microsatellite; primers; sea urchin; Strongylocentrotus spp;
D O I
10.1046/j.1471-8286.2002.00295.x
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
We present six dinucleotide repeats that were developed and characterized in Strongylocentrotus droebachiensis and also tested in S. purpuratus. Four of these loci are polymorphic in S. droebachiensis (13-20 alleles, N = 100) and five are polymorphic in S. purpuratus (5-12 alleles, N = 10). We are currently using these markers to investigate the population substructure of shallow water populations of S. droebachiensis in the north Atlantic.
引用
收藏
页码:493 / 494
页数:2
相关论文
共 7 条
[1]  
Ausubel F.A., 1999, CURRENT PROTOCOLS MO
[2]   Microsatellite loci in wild-type and inbred Strongylocentrotus purpuratus [J].
Cameron, RA ;
Leahy, PS ;
Britten, RJ ;
Davidson, EH .
DEVELOPMENTAL BIOLOGY, 1999, 208 (02) :255-264
[3]  
GROSBERG RK, 1996, MOL ZOOLOGY ADV STRA, P470
[4]  
Hatcher Bruce G., 1997, Bulletin of the Aquaculture Association of Canada, V97, P62
[5]  
Perrin C, 2000, MOL ECOL, V9, P2221
[6]   GENEPOP (VERSION-1.2) - POPULATION-GENETICS SOFTWARE FOR EXACT TESTS AND ECUMENICISM [J].
RAYMOND, M ;
ROUSSET, F .
JOURNAL OF HEREDITY, 1995, 86 (03) :248-249
[7]   Recurrent outbreaks of disease in sea urchins Strongylocentrotus droebachiensis in Nova Scotia: Evidence for a link with large-scale meteorologic and oceanographic events [J].
Scheibling, RE ;
Hennigar, AW .
MARINE ECOLOGY PROGRESS SERIES, 1997, 152 (1-3) :155-165