Evaluation of the isoflavone and total phenolic contents of kefir-fermented soymilk storage and after the in vitro digestive system simulation

被引:61
作者
Fernandes, Meg da Silva [1 ]
Lima, Fernando Sanches [1 ]
Rodrigues, Daniele [1 ]
Handa, Cintia [1 ]
Guelfi, Marcela [1 ]
Garcia, Sandra [1 ]
Ida, Elza Iouko [1 ]
机构
[1] Univ Estadual Londrina, Dept Food Sci & Technol, BR-86051990 Londrina City, Parana, Brazil
关键词
Aglycone; Kefir; Phenolic compounds; Functional; In vitro digestive system; LACTOBACILLUS-ACIDOPHILUS LA-5; BETA-GLUCOSIDASE; ANTIOXIDANT ACTIVITY; SOYBEAN FLOUR; CANCER CELLS; SOY PRODUCT; MILK; PHYTOESTROGENS; TRANSFORMATION; HYDROLYSIS;
D O I
10.1016/j.foodchem.2017.02.095
中图分类号
O69 [应用化学];
学科分类号
081704 ;
摘要
This study aimed to evaluate the isoflavone and total phenolic contents in kefir-fermented soymilk storage and after the in vitro digestive system simulation (DSS). Soymilk was fermented with kefir culture (0.02 UC/L) at 25 degrees C for 15 h and stored at 4 degrees C for 4 days. After the fermentation and storage, the isoflavone and total phenolic contents were quantified by high performance liquid chromatography and spectrophotometry, respectively. The cell viability of lactic acid bacteria and yeast was evaluated. Fermentation promoted an increase of approximately 3 log CFU/g cycles of the microorganisms and the storage process did not alter the aglycone isoflavones and total phenolic contents. The content of aglycone isoflavones increased 2-fold, and the total phenolic content increased 9-fold. Therefore, kefir-fermented soymilk is a good source of aglycone isoflavones and phenolics, since the content of these substances was increased significantly after the in vitro digestive system simulation of the product. (C) 2017 Elsevier Ltd. All rights reserved.
引用
收藏
页码:373 / 380
页数:8
相关论文
共 38 条
[1]   The effect of thermal treatment of whole soybean flour on the conversion of isoflavones and inactivation of trypsin inhibitors [J].
Andrade, J. C. ;
Mandarino, J. M. G. ;
Kurozawa, L. E. ;
Ida, E. I. .
FOOD CHEMISTRY, 2016, 194 :1095-1101
[2]  
Baú TR, 2015, INT J FOOD SCI NUTR, V66, P845, DOI [10.3109/09637486.2015.1095861, 10.3109/09637486.2015]
[3]   Soymilk processing with higher isoflavone aglycone content [J].
Bau, T. R. ;
Ida, E. I. .
FOOD CHEMISTRY, 2015, 183 :161-168
[4]   Evaluation of a Functional Soy Product with Addition of Soy Fiber and Fermented With Probiotic Kefir Culture [J].
Bau, Tahis Regina ;
Garcia, Sandra ;
Ida, Elza Iouko .
BRAZILIAN ARCHIVES OF BIOLOGY AND TECHNOLOGY, 2014, 57 (03) :402-409
[5]   Impact of inulin and okara on Lactobacillus acidophilus La-5 and Bifidobacterium animalis Bb-12 viability in a fermented soy product and probiotic survival under in vitro simulated gastrointestinal conditions [J].
Bedani, Raquel ;
Rossi, Elizeu Antonio ;
Isay Saad, Susana Marta .
FOOD MICROBIOLOGY, 2013, 34 (02) :382-389
[6]  
BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3
[7]  
Brasil. Agencia Nacional de Vigilancia Sanitaria. (ANVISA), 2016, INF TECN PROB ASS IM
[8]   Transformation of isoflavone phytoestrogens during the fermentation of soymilk with lactic acid bacteria and bifidobacteria [J].
Chien, Hsiang-Lin ;
Huang, Hui-Yu ;
Chou, Cheng-Chun .
FOOD MICROBIOLOGY, 2006, 23 (08) :772-778
[9]   Effect of processing, fermentation, and aging treatment to content and profile of phenolic compounds in soybean seed, soy curd and soy paste [J].
Chung, Ill-Min ;
Seo, Su-Hyun ;
Ahn, Joung-Kuk ;
Kim, Seung-Hyun .
FOOD CHEMISTRY, 2011, 127 (03) :960-967
[10]   Optimisation of soybean hydrothermal treatment for the conversion of β-glucoside isoflavones to aglycones [J].
de Lima, Fernando Sanches ;
Ida, Elza Iouko .
LWT-FOOD SCIENCE AND TECHNOLOGY, 2014, 56 (02) :232-239