In vitro micropropagation of Primula scotica:: a rare Scottish plant

被引:46
作者
Benson, EE [1 ]
Danaher, JE [1 ]
Pimbley, IM [1 ]
Anderson, CT [1 ]
Wake, JE [1 ]
Daley, S [1 ]
Adams, LK [1 ]
机构
[1] Univ Abertay Dundee, Sch Sci & Engn, Div Mol & Life Sci, Plant Conservat Biotechnol Grp, Dundee DD1 1HG, Scotland
关键词
conservation biotechnology; endangered species; ex situ conservation; micropropagation; Primula scotica; Primulacae;
D O I
10.1023/A:1008941726419
中图分类号
X176 [生物多样性保护];
学科分类号
090705 ;
摘要
A simple micropropagation method is reported for Primula scotica, a rare plant, endemic to the North of Scotland. The technique involves the clonal proliferation of seed-derived plantlets on either hormone free tissue culture medium or on medium containing benzyl amino purine and indole acetic acid. Average multiplication rates of 4-6 were obtained for plants grown on plant growth regulator supplemented medium. The micropropagation method was applied to four different clones of P. scotica and clonal differences were observed in relation to media type. Some plants from certain clones did display hyperhydricity, however, this was circumvented by using frequent sub-culturing intervals and transferring the plants to hormone-free medium. Plantlets rooted on both media types and displayed normal, true-to-type rosette morphology. The cultures did not callus and development proceeded via shoot and root production only. The in vitro-grown plants could be transferred to ex vitro conditions and a range of growth substrates were assessed for their efficacy in supporting ex vitro growth, with a view to developing longer-term strategies for the transfer and reintroduction of micropropagated P. scotica plants into natural habitats. The simple method described in this paper may offer the potential of being applied to other endangered Primula spp.
引用
收藏
页码:711 / 726
页数:16
相关论文
共 15 条
[1]  
[Anonymous], 1985, NATURAL HIST ORKNEY
[2]  
Benson E., 1999, PLANT CONSERVATION B
[3]  
BENSON EE, 1998, IN VITRO CELL DEV, V34, pA61
[4]   SURVIVAL AND FLOWERING OF PRIMULA-SCOTICA HOOK [J].
BULLARD, ER ;
SHEARER, HDH ;
DAY, JD ;
CRAWFORD, RMM .
JOURNAL OF ECOLOGY, 1987, 75 (03) :589-602
[5]  
Edson JL, 1996, IN VITRO CELL DEV-PL, V32, P267
[6]  
FAY MF, 1992, IN VITRO CELL DEV-PL, V28P, P1
[7]   LOW GENETIC DIVERSITY IN THE SCOTTISH ENDEMIC PRIMULA-SCOTICA HOOK [J].
GLOVER, BJ ;
ABBOTT, RJ .
NEW PHYTOLOGIST, 1995, 129 (01) :147-153
[8]  
Gonzalez-Benito M. E., 1994, Botanic Gardens Micropropagation News, V1, P82
[9]  
Gonzalez-Benito ME, 1999, PLANT CONSERVATION B, P251
[10]   The effect of ventilation rate on proliferation and hyperhydricity of Dianthus caryophyllus L. [J].
Majada J.P. ;
Fal M.A. ;
Sánchez-TaméS R. .
In Vitro Cellular & Developmental Biology - Plant, 1997, 33 (1) :62-69