A fluorescence based immunochromatographic sensor for monitoring chlorpheniramine and its comparison with a gold nanoparticle-based lateral-flow strip

被引：1

作者：

Zhou, Shengyang ^{[1
,2
,3
]}

Xu, Xinxin ^{[1
,2
,3
]}

Wang, Li ^{[1
,2
,3
]}

Guo, Lingling ^{[1
,2
,3
]}

Liu, Liqiang ^{[1
,2
,3
]}

Kuang, Hua ^{[1
,2
,3
]}

Xu, Chuanlai ^{[1
,2
,3
]}

机构：

[1] Jiangnan Univ, State Key Lab Food Sci & Technol, Wuxi, Jiangsu, Peoples R China

[2] Jiangnan Univ, Int Joint Res Lab Biointerface & Biodetect, Wuxi, Jiangsu, Peoples R China

[3] Jiangnan Univ, Sch Food Sci & Technol, Wuxi, Jiangsu, Peoples R China

来源：

ANALYST | 2021年 / 146卷 / 11期

关键词：

PERFORMANCE LIQUID-CHROMATOGRAPHY; MONOCLONAL-ANTIBODY; HUMAN PLASMA; QUANTITATIVE DETECTION; RAPID DETECTION; IC-ELISA; ASSAY; IMMUNOASSAY; MALEATE; QUANTIFICATION;

D O I：

10.1039/d1an00423a

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Chlorpheniramine (CPM) is an illegal additive found in herbal teas and health foods, and its excessive intake can cause health problems. In this study, a CPM monoclonal antibody (mAb) was developed based on a new type of hapten. The mAb was found to belong to the IgG2b subclass and showed high sensitivity and specificity when used in ELISA, with a half-maximal inhibitory concentration (IC50) of 0.98 ng mL(-1) and cross-reactivity (CR) values below 1.8% when compared to antiallergic drugs. Based on the mAb produced, a fluorescent microsphere-based immunochromatographic strip assay (FM-ICS) and a gold nanoparticle-based immunochromatographic strip assay (GNP-ICS) were developed for the rapid and sensitive detection of CPM in herbal tea samples. Under optimal conditions, the cut-off values for the FM-ICS and GNP-ICS were 10 ng mL(-1) and 100 ng mL(-1), respectively, in herbal tea samples. The FM-ICS exhibited a higher sensitivity than GNP-ICS, but both could produce results within 15 min. In addition, a variety of high-throughput rapid immunoassay formats could be implemented based on this mAb for use as a convenient and reliable tool for the determination of CPM exposure in foods and the environment.

引用

页码：3589 / 3598

页数：10

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