Seed lipoxygenase products modulate Aspergillus mycotoxin biosynthesis

被引:135
作者
Burow, GB
Nesbitt, TC
Dunlap, J
Keller, NP
机构
[1] TEXAS A&M UNIV,DEPT PLANT PATHOL & MICROBIOL,COLLEGE STN,TX 77843
[2] TEXAS A&M UNIV,TEXAS AGR EXPT STN,WESLACO,TX 78593
关键词
aflatoxin B-1; 13S-hydroperoxy linolenic acid; methyl jasmonate; mRNA accumulation; plant-microbe interactions; stcU; ver-1;
D O I
10.1094/MPMI.1997.10.3.380
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Oilseed crops are frequently subject to contamination by mycotoxins produced by Aspergillus spp., particularly aflatoxin (AF) and to a lesser extent sterigmatocystin (ST). Several studies have suggested that metabolites generated from the plant lipoxygenase (LOX) pathway may either decrease or increase mycotoxin production by Aspergillus spp. We tested the possibility that the occurrence of seed LOX isozymes that produce distinct hydroperoxy fatty acids may account for these different effects on AF biosynthesis, For example, soybean LOX1 catalyzes the addition of O-2 to the C13 position of linoleic and linolenic acids while maize embryo LOX catalyzes the addition of O-2 to the C9 position. In vitro experiments showed that 13S-hydroperoxy fatty acids at concentrations of 10 and 100 mu M repressed AF and ST pathway gene expression and significantly (P = 0.05) reduced AF and ST production in both A. parasiticus (AF producer) and A. nidulans (ST producer). Treatment with 1 mu M 13S-hydroperoxy linoleic acid also significantly decreased AF production when introduced into growth media at continuous 24-h intervals. In contrast, the same concentrations of 9S-hydroperoxy linoleic acid did not reduce AF or ST production but extended the length of time AF and ST transcripts were detectable. These results show that 13S-hydroperoxy fatty acids directly or indirectly repress AF and ST biosynthesis and provide in vitro evidence that specific seed lipoxygenase activity could provide resistance to mycotoxin contamination by Aspergillus spp.
引用
收藏
页码:380 / 387
页数:8
相关论文
共 53 条
[1]   INCORPORATION OF LABELLED COMPOUNDS INTO AFLATOXINS [J].
ADYE, J ;
MATELES, RI .
BIOCHIMICA ET BIOPHYSICA ACTA, 1964, 86 (02) :418-&
[2]  
Aziz NH, 1995, MICROBIOS, V84, P29
[3]  
Bewley J.D., 1985, SEEDS PHYSL DEV GERM
[4]   Twenty-five coregulated transcripts define a sterigmatocystin gene cluster in Aspergillus nidulans [J].
Brown, DW ;
Yu, JH ;
Kelkar, HS ;
Fernandes, M ;
Nesbitt, TC ;
Keller, NP ;
Adams, TH ;
Leonard, TJ .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1996, 93 (04) :1418-1422
[5]   SPREAD OF ASPERGILLUS-FLAVUS IN COTTON BOLLS, DECAY OF INTERCAPILLARY MEMBRANES, AND PRODUCTION OF FUNGAL PECTINASES [J].
BROWN, RL ;
CLEVELAND, TE ;
COTTY, PJ ;
MELLON, JE .
PHYTOPATHOLOGY, 1992, 82 (04) :462-467
[6]   LIVING MAIZE EMBRYO INFLUENCES ACCUMULATION OF AFLATOXIN IN MAIZE KERNELS [J].
BROWN, RL ;
COTTY, PJ ;
CLEVELAND, TE ;
WIDSTROM, NW .
JOURNAL OF FOOD PROTECTION, 1993, 56 (11) :967-971
[7]   REGULATION OF AFLATOXIN BIOSYNTHESIS - EFFECT OF GLUCOSE ON ACTIVITIES OF VARIOUS GLYCOLYTIC-ENZYMES [J].
BUCHANAN, RL ;
LEWIS, DF .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1984, 48 (02) :306-310
[8]   ISOLATION OF A SEXUAL SPORULATION HORMONE FROM ASPERGILLUS-NIDULANS [J].
CHAMPE, SP ;
ELZAYAT, AAE .
JOURNAL OF BACTERIOLOGY, 1989, 171 (07) :3982-3988
[9]   INCREASED EXPRESSION OF ASPERGILLUS-PARASITICUS AFLR, ENCODING A SEQUENCE-SPECIFIC DNA-BINDING PROTEIN, RELIEVES NITRATE INHIBITION OF AFLATOXIN BIOSYNTHESIS [J].
CHANG, PK ;
EHRLICH, KC ;
YU, JJ ;
BHATNAGAR, D ;
CLEVELAND, TE .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1995, 61 (06) :2372-2377
[10]   VARIATION IN POLYGALACTURONASE PRODUCTION AMONG ASPERGILLUS-FLAVUS ISOLATES [J].
COTTY, PJ ;
CLEVELAND, TE ;
BROWN, RL ;
MELLON, JE .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1990, 56 (12) :3885-3887