SARS-CoV-2 infection elicits a rapid neutralizing antibody response that correlates with disease severity

被引：73

作者：

Trinite, Benjamin ^{[1
]}

Tarres-Freixas, Ferran ^{[1
]}

Rodon, Jordi ^{[2
]}

Pradenas, Edwards ^{[1
]}

Urrea, Victor ^{[1
]}

Marfl, Silvia ^{[1
]}

Rodriguez de la Concepcion, Maria Luisa ^{[1
]}

Avila-Nieto, Carlos ^{[1
]}

Aguilar-Gurrieri, Carmen ^{[1
]}

Barajas, Ana ^{[1
]}

Ortiz, Raquel ^{[1
]}

Paredes, Roger ^{[1
,3
]}

Mateu, Lourdes ^{[3
]}

Valencia, Alfonso ^{[4
]}

Guallar, Victor ^{[4
,5
]}

Ruiz, Lidia ^{[1
]}

Grau, Eulalia ^{[1
]}

Massanella, Marta ^{[1
]}

Puig, Jordi ^{[3
]}

Chamorro, Anna ^{[3
]}

Izquierdo-Useros, Nuria ^{[1
]}

Segales, Joaquim ^{[2
,6
]}

Clotet, Bonaventura ^{[1
,3
,7
]}

Carrillo, Jorge ^{[1
]}

Vergara-Alert, Julia ^{[2
]}

Blanco, Julia ^{[1
,7
]}

机构：

[1] Hosp Badalona Germans Trias & Pujol, Germans Trias & Pujol Res Inst IGTP, Inst Recerca La Sida, IrsiCaixa AIDS Res Inst, Can Ruti Campus,Ctra Canyet S-N, Badalona 08916, Catalonia, Spain

[2] IRTA UAB, IRTA Ctr Recerca Sanitat Anim CReSA, Campus UAB, Bellaterra 08193, Catalonia, Spain

[3] Germans Trias & Pujol Hosp, Fight AIDS Fdn FLS, Infect Dis Dept, Badalona, Catalonia, Spain

[4] Barcelona Supercomp Ctr, Barcelona, Catalonia, Spain

[5] Catalan Inst Res & Adv Studies ICREA, Barcelona, Catalonia, Spain

[6] CReSA IRTA UAB, UAB, Campus UAB, Cerdanyola Del Valles 08193, Catalonia, Spain

[7] Univ Vic Cent Univ Catalonia UVic UCC, Victoria, Catalonia, Australia

来源：

SCIENTIFIC REPORTS | 2021年 / 11卷 / 01期

关键词：

D O I：

10.1038/s41598-021-81862-9

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The protective effect of neutralizing antibodies in SARS-CoV-2 infected individuals is not yet well defined. To address this issue, we have analyzed the kinetics of neutralizing antibody responses and their association with disease severity. Between March and May 2020, the prospective KING study enrolled 72 COVID-19+ participants grouped according to disease severity. SARS-CoV-2 infection was diagnosed by serological and virological tests. Plasma neutralizing responses were assessed against replicative virus and pseudoviral particles. Multiple regression and non-parametric tests were used to analyze dependence of parameters. The magnitude of neutralizing titers significantly increased with disease severity. Hospitalized individuals developed higher titers compared to mild-symptomatic and asymptomatic individuals, which together showed titers below the detection limit in 50% of cases. Longitudinal analysis confirmed the strong differences in neutralizing titers between non-hospitalized and hospitalized participants and showed rapid kinetics of appearance of neutralizing antibodies (50% and 80% of maximal activity reached after 11 and 17 days after symptoms onset, respectively) in hospitalized patients. No significant impact of age, gender or treatment on the neutralizing titers was observed in this limited cohort. These data identify a clear association of humoral immunity with disease severity and point to immune mechanisms other than antibodies as relevant players in COVID-19 protection.

引用

页数：10

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