A transgenic DND1GFP fusion allele reports in vivo expression and RNA-binding targets in undifferentiated mouse germ cells

被引:10
作者
Ruthig, Victor A. [1 ]
Yokonishi, Tetsuhiro [1 ]
Friedersdorf, Matthew B. [2 ]
Batchvarova, Sofia [1 ]
Hardy, Josiah [1 ]
Garness, Jason A. [1 ]
Keene, Jack D. [2 ]
Capel, Blanche [1 ]
机构
[1] Duke Univ, Med Ctr, Dept Cell Biol, Durham, NC 27710 USA
[2] Duke Univ, Med Ctr, Dept Mol Genet & Microbiol, Durham, NC USA
关键词
RNA immunoprecipitation; male germ cell; dead end 1; RNA-binding protein; spermatogonia; meiosis; MESSENGER-RNA; DEAD-END; TESTICULAR TERATOMAS; DIFFERENTIAL-DIAGNOSIS; PROTEIN NANOS2; STEM-CELLS; P-BODIES; C-KIT; IDENTIFICATION; SPERMATOGENESIS;
D O I
10.1093/biolre/ioaa233
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
In vertebrates, the RNA-binding protein (RBP) dead end 1 (DND1) is essential for primordial germ cell (PGC) survival and maintenance of cell identity. In multiple species, Dnd1 loss or mutation leads to severe PGC loss soon after specification or, in some species, germ cell transformation to somatic lineages. Our investigations into the role of DND1 in PGC specification and differentiation have been limited by the absence of an available antibody. To address this problem, we used CRISPR/Cas9 gene editing to establish a transgenic mouse line carrying a DND1(GFP) fusion allele. We present imaging analysis of DND1(GFP) expression showing that DND1(GFP) expression is heterogeneous among male germ cells (MGCs) and female germ cells (FGCs). DND1(GFP) was detected in MGCs throughout fetal life but lost from FGCs at meiotic entry. In postnatal and adult testes, DND1(GFP) expression correlated with classic markers for the premeiotic spermatogonial population. Utilizing the GFP tag for RNA immunoprecipitation (RIP) analysis in MGCs validated this transgenic as a tool for identifying in vivo transcript targets of DND1. The DND1(GFP) mouse line is a novel tool for isolation and analysis of embryonic and fetal germ cells, and the spermatogonial population of the postnatal and adult testis. Summary sentence Characterization of this novel transgenic mouse showed that DND1GFP is expressed throughout the pre-meiotic germline, supports growing evidence in the field of germ cell heterogeneity, and demonstrated the tagged allele can be used for identification of DND1 targets.
引用
收藏
页码:861 / 874
页数:14
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