Decidual NK cells alter in vitro first trimester extravillous cytotrophoblast migration:: A role for IFN-γ

被引:112
作者
Hu, Yuxiang
Dutz, Jan P.
MacCalman, Colin D.
Yong, Paul
Tan, Rusung
von Dadelszen, Peter
机构
[1] Univ British Columbia, Dept Obstet & Gynaecol, Vancouver, BC V6H 3N1, Canada
[2] Univ British Columbia, Dept Med, Vancouver, BC V6H 3N1, Canada
[3] Univ British Columbia, Dept Pathol & Lab Med, Vancouver, BC V6H 3N1, Canada
[4] Univ British Columbia, Dept Med Genet, Vancouver, BC V6H 3N1, Canada
[5] Ctr Healthcare Innovat & Improvement, Child & Family Res Inst, Vancouver, BC, Canada
[6] Program Infect & Immun, Vancouver, BC, Canada
[7] Program Reprod Hlth, Vancouver, BC, Canada
关键词
NATURAL-KILLER-CELLS; MATRIX METALLOPROTEINASES; TROPHOBLAST MIGRATION; PREGNANCY DECIDUA; INTERFERON-GAMMA; INVASIVE PATHWAY; HLA-E; DIFFERENTIATION; LYMPHOCYTES; INHIBITION;
D O I
10.4049/jimmunol.177.12.8522
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Abnormal placentation results in either inadequate (consequences: recurrent miscarriage, intrauterine growth restriction, and preeclampsia) or overzealous (consequences: placenta accreta, increta, and percreta) placentation. NK cells dominate in first trimester decidua and probably control extravillous cytotrophoblast (EVT) invasion. We examined this interaction in a novel way, using NK cells and villous explants from concordant first trimester pregnancies cocultured using a new collagen (two-dimensional) model of placentation. Decidual NK (dNK) cells exerted contact-independent inhibition of normal cytotrophoblast migration, associated with changes in the cytotrophoblast expression of metalloproteases-2 and -9, and plasminogen activator inhibitor-1. dNK cells did not affect EVT proliferation and apoptosis, and cell column formation. dNK cell effects were partially reversed by neutralizing Abs against IFN-gamma. We provide ex vivo human evidence of a direct role for dNK in modulating EVT differentiation as they form columns and then migrate from anchoring villi.
引用
收藏
页码:8522 / 8530
页数:9
相关论文
共 47 条
[1]  
Albertsson P, 2000, IN VIVO, V14, P269
[2]   Development of cytotrophoblast columns from explanted first-trimester human placental villi:: Role of fibronectin and integrinα5β1 [J].
Aplin, JD ;
Haigh, T ;
Jones, CJP ;
Church, HJ ;
Vicovac, L .
BIOLOGY OF REPRODUCTION, 1999, 60 (04) :828-838
[3]   EXPRESSION OF INTEGRIN ALPHA-6-BETA-4 IN HUMAN TROPHOBLAST AND ITS LOSS FROM EXTRAVILLOUS CELLS [J].
APLIN, JD .
PLACENTA, 1993, 14 (02) :203-215
[4]   Assessment of requirements for IL-15 and IFN regulatory factors in uterine NK cell differentiation and function during pregnancy [J].
Ashkar, AA ;
Black, GP ;
Wei, QX ;
He, H ;
Liang, LC ;
Head, JR ;
Croy, BA .
JOURNAL OF IMMUNOLOGY, 2003, 171 (06) :2937-2944
[5]   Tumor necrosis factor-α inhibits trophoblast migration through elevation of plasminogen activator inhibitor-1 in first-trimester villous explant cultures [J].
Bauer, S ;
Pollheimer, J ;
Hartmann, J ;
Husslein, P ;
Aplin, JD ;
Knöfler, M .
JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM, 2004, 89 (02) :812-822
[6]   Kinetics of hepatitis B surface antigen-specific immune responses in acute and chronic hepatitis B or after HBs vaccination:: Stimulation of the in vitro antibody response by interferon gamma [J].
Böcher, WO ;
Herzog-Hauff, S ;
Schlaak, J ;
zum Büschenfelde, KHM ;
Löhr, HF .
HEPATOLOGY, 1999, 29 (01) :238-244
[7]   IMMUNE ASPECTS OF PATHOLOGY OF THE PLACENTAL BED CONTRIBUTING TO PREGNANCY PATHOLOGY [J].
BULMER, JN .
BAILLIERES CLINICAL OBSTETRICS AND GYNAECOLOGY, 1992, 6 (03) :461-488
[8]   Endoglin regulates trophoblast differentiation along the invasive pathway in human placental villous explants [J].
Caniggia, I ;
Taylor, CV ;
Ritchie, JWK ;
Lye, SJ ;
Letarte, M .
ENDOCRINOLOGY, 1997, 138 (11) :4977-4988
[9]   CD56(+) LYMPHOID-CELLS IN HUMAN 1ST-TRIMESTER PREGNANCY DECIDUA AS A SOURCE OF NOVEL TRANSFORMING GROWTH FACTOR-BETA(2)-RELATED IMMUNOSUPPRESSIVE FACTORS [J].
CLARK, DA ;
VINCE, G ;
FLANDERS, KC ;
HIRTE, H ;
STARKEY, P .
HUMAN REPRODUCTION, 1994, 9 (12) :2270-2277
[10]   Uterine natural killer cells: insights into their cellular and molecular biology from mouse modelling [J].
Croy, BA ;
He, H ;
Esadeg, S ;
Wei, QX ;
McCartney, D ;
Zhang, JH ;
Borzychowski, A ;
Ashkar, AA ;
Black, GP ;
Evans, SS ;
Chantakru, S ;
van den Heuvel, M ;
Paffaro, VA ;
Yamada, AT .
REPRODUCTION, 2003, 126 (02) :149-160