Genome-Wide Identification and Low Temperature Responsive Pattern of Actin Depolymerizing Factor (ADF) Gene Family in Wheat (Triticum aestivum L.)

被引:20
作者
Xu, Ke [1 ]
Zhao, Yong [1 ]
Zhao, Sihang [1 ]
Liu, Haodong [1 ]
Wang, Weiwei [1 ,2 ]
Zhang, Shuhua [1 ]
Yang, Xueju [1 ]
机构
[1] Hebei Agr Univ, State Key Lab North China Crop Improvement & Regu, Baoding, Peoples R China
[2] Cangzhou Acad Agr & Forestry Sci, Cangzhou, Peoples R China
来源
FRONTIERS IN PLANT SCIENCE | 2021年 / 12卷
关键词
actin depolymerizing factor; wheat (Triticum aestivum L; low temperature; genome-wide identification; transgenic Arabidopsis; ORGANIZATION; CYTOSKELETON; EXPRESSION; FILAMENTS; RESISTANCE; PROTEINS;
D O I
10.3389/fpls.2021.618984
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
The actin depolymerizing factor (ADF) gene family, which is conserved in eukaryotes, is important for plant development, growth, and stress responses. Cold stress restricts wheat growth, development, and distribution. However, genome-wide identification and functional analysis of the ADF family in wheat is limited. Further, because of the promising role of ADF genes in cold response, there is need for an understanding of the function of this family on wheat under cold stress. In this study, 25 ADF genes (TaADFs) were identified in the wheat genome and they are distributed on 15 chromosomes. The TaADF gene structures, duplication events, encoded conversed motifs, and cis-acting elements were investigated. Expression profiles derived from RNA-seq data and real-time quantitative PCR analysis revealed the tissue- and temporal-specific TaADF expression patterns. In addition, the expression levels of TaADF13/16/17/18/20/21/22 were significantly affected by cold acclimation or freezing conditions. Overexpression of TaADF16 increased the freezing tolerance of transgenic Arabidopsis, possibly because of enhanced ROS scavenging and changes to the osmotic regulation in cells. The expression levels of seven cold-responsive genes were up-regulated in the transgenic Arabidopsis plants, regardless of whether the plants were exposed to low temperature. These findings provide fundamental information about the wheat ADF genes and may help to elucidate the regulatory effects of the encoded proteins on plant development and responses to low-temperature stress.
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页数:14
相关论文
共 55 条
[1]   Actin modulates shape and mechanics of tubular membranes [J].
Allard, A. ;
Bouzid, M. ;
Betz, T. ;
Simon, C. ;
Abou-Ghali, M. ;
Lemiere, J. ;
Valentino, F. ;
Manzi, J. ;
Brochard-Wyart, F. ;
Guevorkian, K. ;
Plastino, J. ;
Lenz, M. ;
Campillo, C. ;
Sykes, C. .
SCIENCE ADVANCES, 2020, 6 (17)
[2]   ExPASy: SIB bioinformatics resource portal [J].
Artimo, Panu ;
Jonnalagedda, Manohar ;
Arnold, Konstantin ;
Baratin, Delphine ;
Csardi, Gabor ;
de Castro, Edouard ;
Duvaud, Severine ;
Flegel, Volker ;
Fortier, Arnaud ;
Gasteiger, Elisabeth ;
Grosdidier, Aurelien ;
Hernandez, Celine ;
Ioannidis, Vassilios ;
Kuznetsov, Dmitry ;
Liechti, Robin ;
Moretti, Sebastien ;
Mostaguir, Khaled ;
Redaschi, Nicole ;
Rossier, Gregoire ;
Xenarios, Ioannis ;
Stockinger, Heinz .
NUCLEIC ACIDS RESEARCH, 2012, 40 (W1) :W597-W603
[3]   PARTIAL-PURIFICATION AND CHARACTERIZATION OF AN ACTIN DEPOLYMERIZING FACTOR FROM BRAIN [J].
BAMBURG, JR ;
HARRIS, HE ;
WEEDS, AG .
FEBS LETTERS, 1980, 121 (01) :178-182
[4]   ACTIN DEPOLYMERIZING FACTOR9 controls development and gene expression in Arabidopsis [J].
Burgos-Rivera, Brunilis ;
Ruzicka, Daniel R. ;
Deal, Roger B. ;
McKinney, Elizabeth C. ;
King-Reid, Lori ;
Meagher, Richard B. .
PLANT MOLECULAR BIOLOGY, 2008, 68 (06) :619-632
[5]   The roles of segmental and tandem gene duplication in the evolution of large gene families in Arabidopsis thaliana [J].
Cannon S.B. ;
Mitra A. ;
Baumgarten A. ;
Young N.D. ;
May G. .
BMC Plant Biology, 4 (1)
[6]   The regulation of actin organization by actin-depolymerizing factor in elongating pollen tubes [J].
Chen, CY ;
Wong, EI ;
Vidali, L ;
Estavillo, A ;
Hepler, PK ;
Wu, HM ;
Cheung, AY .
PLANT CELL, 2002, 14 (09) :2175-2190
[7]   Plant-mPLoc: A Top-Down Strategy to Augment the Power for Predicting Plant Protein Subcellular Localization [J].
Chou, Kuo-Chen ;
Shen, Hong-Bin .
PLOS ONE, 2010, 5 (06)
[8]   Actin depolymerizing factors ADF7 and ADF10 play distinct roles during pollen development and pollen tube growth [J].
Daher, Firas Bou ;
Geitmann, Anja .
PLANT SIGNALING & BEHAVIOR, 2012, 7 (07) :879-881
[9]   Identification and characterization of a low temperature regulated gene encoding an actin-binding protein from wheat [J].
Danyluk, J ;
Carpentier, E ;
Sarhan, F .
FEBS LETTERS, 1996, 389 (03) :324-327
[10]   ADF proteins are involved in the control of flowering and regulate F-actin organization, cell expansion, and organ growth in Arabidopsis [J].
Dong, CH ;
Xia, GX ;
Hong, Y ;
Ramachandran, S ;
Kost, B ;
Chua, NH .
PLANT CELL, 2001, 13 (06) :1333-1346