UBE2T knockdown inhibits gastric cancer progression

被引：60

作者：

Luo, Changjiang ^{[1
,2
]}

Yao, Yunyi ^{[3
,4
]}

Yu, Zeyuan ^{[1
,2
]}

Zhou, Huinian ^{[1
,2
]}

Guo, Lingyun ^{[1
,2
]}

Zhang, Junqiang ^{[1
,2
]}

Cao, Hongtai ^{[1
,2
]}

Zhang, Genyuan ^{[1
,2
]}

Li, Yumin ^{[1
,2
]}

Jiao, Zuoyi ^{[1
,2
]}

机构：

[1] Lanzhou Univ, Hosp 2, Dept Gen Surg, Lanzhou 730030, Gansu, Peoples R China

[2] Key Lab Digest Syst Tumors Gansu Prov, Lanzhou 730030, Gansu, Peoples R China

[3] Suzhou Vocat Hlth Coll, Dept Med Technol, Suzhou 215009, Jiangsu, Peoples R China

[4] Suzhou Vocat Hlth Coll, Key Lab Biotechnol Lab Med Suzhou, Suzhou 215009, Jiangsu, Peoples R China

来源：

ONCOTARGET | 2017年 / 8卷 / 20期

关键词：

UBE2T; ubiquitination; gastric cancer; tumor apoptosis; invasion and metastasis; ELEVATED EXPRESSION; UBIQUITIN LIGASES; CYCLIN D1; CELLS; MYC; DEUBIQUITINASES; DEGRADATION; PROTEASOME; GSK3-BETA; INVASION;

D O I：

10.18632/oncotarget.15947

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Ubiquitin-conjugating enzymes (E2 enzymes) such as UBE2T target proteins for degradation via the proteasome. Here, we examined the effects of UBE2T on the progression of gastric cancer. UBE2T was highly expressed in gastric tumors and gastric cancer cells. siRNA-mediated suppression of UBE2T inhibited gastric cancer cell proliferation and colony formation by promoting cell cycle arrest at G2/M phase and increasing apoptosis. Suppression of UBE2T also attenuated the invasive and metastatic abilities of gastric cancer cells by altering expression of epithelialmesenchymal transition (EMT)-related factors. A xenograft model in which nude mice were injected with UBE2T knockdown human gastric cancer cells confirmed that suppression of UBE2T also decreased tumor formation and growth in vivo. Expression levels of CCND1, Phospho-GSK3B, WNT family members, and MYC were all affected by UBE2T knockdown. These results suggest that UBE2T plays a critical role in gastric cancer, and that it may serve as a useful prognostic biomarker and therapeutic target in gastric cancer patients.

引用

页码：32639 / 32654

页数：16

共 40 条

[1] Common Food Additive Carrageenan Stimulates Wnt/β-Catenin Signaling in Colonic Epithelium by Inhibition of Nucleoredoxin Reduction [J].