Design of liposomes containing photopolymerizable phospholipids for triggered release of contents

被引：85

作者：

Yavlovich, Amichai ^{[1
]}

Singh, Alok ^{[2
]}

Tarasov, Sergey ^{[3
]}

Capala, Jacek ^{[4
]}

Blumenthal, Robert ^{[1
]}

Puri, Anu ^{[1
]}

机构：

[1] NCI, Membrane Struct & Funct Sect, Ctr Canc Res Nanobiol Program, NIH, Frederick, MD 21702 USA

[2] USN, Res Lab, Ctr Biomol Sci & Engn, Washington, DC 20375 USA

[3] NCI, Struct Biophys Lab, Ctr Canc Res, NIH, Frederick, MD 21702 USA

[4] NCI, Ctr Canc Res, NIH, Bethesda, MD 20892 USA

来源：

JOURNAL OF THERMAL ANALYSIS AND CALORIMETRY | 2009年 / 98卷 / 01期

关键词：

Polymerizable lipids; Lipid packing; Triggered drug release; Diacetylene phospholipids; Light-sensitive liposomes; Lipid modification; Phase separation; POLYMERIZABLE LIPID BILAYERS; THERMOTROPIC PHASE-BEHAVIOR; PHYSICAL-PROPERTIES; ACYL CHAINS; PHOSPHATIDYLCHOLINES; MEMBRANES; MIXTURES; MONOLAYERS; VESICLES; DELIVERY;

D O I：

10.1007/s10973-009-0228-8

中图分类号：

O414.1 [热力学];

学科分类号：

摘要：

We describe a novel class of light-triggerable liposomes prepared from a photo-polymerizable phospholipid DC8,9PC (1,2-bis (tricosa-10,12-diynoyl)-sn-glycero-3-phosphocholine) and DPPC (1,2-Dipalmitoyl-sn-Glycero-3-Phosphocholine). Exposure to UV (254 nm) radiation for 0-45 min at 25 A degrees C resulted in photo-polymerization of DC8,9PC in these liposomes and the release of an encapsulated fluorescent dye (calcein). Kinetics and extents of calcein release correlated with mol% of DC8,9PC in the liposomes. Photopolymerization and calcein release occurred only from DPPC/DC8,9PC but not from Egg PC/DC8,9PC liposomes. Our data indicate that phase separation and packing of polymerizable lipids in the liposome bilayer are major determinants of photo-activation and triggered contents release.

引用

页码：97 / 104

页数：8

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