Genome-Wide Analysis of Artificial Mutations Induced by Ethyl Methanesulfonate in the Eggplant (Solanum melongena L.)

被引:12
作者
Xiao, Xi-ou [1 ,2 ,3 ]
Lin, Wenqiu [1 ,2 ]
Li, Ke [1 ,2 ]
Feng, Xuefeng [1 ,2 ]
Jin, Hui [1 ,2 ]
Zou, Huafeng [1 ,2 ]
机构
[1] Chinese Acad Trop Agr Sci, South Subtrop Crop Res Inst, Zhanjiang City 524091, Peoples R China
[2] Zhanjiang City Key Lab Trop Crops Genet Improveme, Zhanjiang City 524091, Guangdong, Peoples R China
[3] Room 507,Huxiu Rd 1, Zhanjiang 52491, Guangdong, Peoples R China
基金
中国国家自然科学基金;
关键词
eggplant; ethyl methanesulfonate mutation; fruit color; gene function; whole-genome re-sequencing; ANTHOCYANIN BIOSYNTHESIS; REVEALS;
D O I
10.3390/genes10080595
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
Whole-genome sequences of four EMS (ethyl methanesulfonate)-induced eggplant mutants were analyzed to identify genome-wide mutations. In total, 173.01 GB of paired-end reads were obtained for four EMS-induced mutants and (WT) wild type and 1,076,010 SNPs (single nucleotide polymorphisms) and 183,421 indels were identified. The most common mutation type was C/G to T/A transitions followed by A/T to G/C transitions. The mean densities were one SNP per 1.3 to 2.6 Mb. The effect of mutations on gene function was annotated and only 7.2% were determined to be deleterious. KEGG (Kyoto Encyclopedia of Genes and Genomes) pathway analysis showed 10 and 11 genes, which were nonsynonymous mutation or frameshift deletion in 48-5 and L6-5 involved in the anthocyanin biosynthesis or flavone and flavonol biosynthesis. QRT-PCR results showed that only the Sme2.5_06210.1_g00004.1, which was annotated as UFGT (Flavonoid galactosidase transferase), expression significantly decreased in the L6-5 mutant compared with the WT. Also, the Sme2.5_06210.1_g00004.1 expression was lower in the colorless eggplant compared with colorful eggplant in the natural eggplant cultivar. These results suggest that Sme2.5_06210.1_g00004.1 may play a key role in eggplant anthocyanin synthesis.
引用
收藏
页数:14
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