Inhibition of siRNA binding to a p19 viral suppressor of RNA silencing by cysteine alkylation

被引：18
作者：
Sagan, Selena M.
Koukiekolo, Roger
Rodgers, Elisabeth
Goto, Natalie K.
Pezacki, John Paul
机构：
[1] Natl Res Council Canada, Steacie Inst Mol Sci, Ottawa, ON K1H 0R6, Canada
[2] Univ Ottawa, Dept Chem, Ottawa, ON K1N 6N5, Canada
[3] Univ Ottawa, Dept Biochem Microbiol & Immunol, Ottawa Inst Syst Biol, Ottawa, ON K1H 8M5, Canada
来源：
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2007年 / 46卷 / 12期
关键词：
chemical probes; fluorescence; RNA silencing; RNA-protein interactions; viruses;
D O I：
10.1002/anie.200603284
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
(Figure Presented) Eukaryotes have evolved complex cellular responses to double-stranded RNA. The quantities of short interfering RNA (siRNA) can be determined rapidly by using 96-well arrays of the carnation Italian ringspot virus (CIRV) p19 protein, which binds double-stranded siRNAs with nanomolar affinity and discriminates siRNA according to length. Two compounds were found to inhibit siRNA binding to CIRV p19 by alkylating active-site cysteine residues (see diagram). © 2007 Wiley-VCH Verlag GmbH &. Co. KGaA.
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收藏
页码：2005 / 2009
页数：5
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