Inhibition of siRNA binding to a p19 viral suppressor of RNA silencing by cysteine alkylation

被引：18
作者：
Sagan, Selena M.
Koukiekolo, Roger
Rodgers, Elisabeth
Goto, Natalie K.
Pezacki, John Paul
机构：
[1] Natl Res Council Canada, Steacie Inst Mol Sci, Ottawa, ON K1H 0R6, Canada
[2] Univ Ottawa, Dept Chem, Ottawa, ON K1N 6N5, Canada
[3] Univ Ottawa, Dept Biochem Microbiol & Immunol, Ottawa Inst Syst Biol, Ottawa, ON K1H 8M5, Canada
来源：
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2007年 / 46卷 / 12期
关键词：
chemical probes; fluorescence; RNA silencing; RNA-protein interactions; viruses;
D O I：
10.1002/anie.200603284
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
(Figure Presented) Eukaryotes have evolved complex cellular responses to double-stranded RNA. The quantities of short interfering RNA (siRNA) can be determined rapidly by using 96-well arrays of the carnation Italian ringspot virus (CIRV) p19 protein, which binds double-stranded siRNAs with nanomolar affinity and discriminates siRNA according to length. Two compounds were found to inhibit siRNA binding to CIRV p19 by alkylating active-site cysteine residues (see diagram). © 2007 Wiley-VCH Verlag GmbH &. Co. KGaA.
引用
收藏
页码：2005 / 2009
页数：5
相关论文
共 14 条
[1]   Role for a bidentate ribonuclease in the initiation step of RNA interference [J].
Bernstein, E ;
Caudy, AA ;
Hammond, SM ;
Hannon, GJ .
NATURE, 2001, 409 (6818) :363-366
[2]   Plants combat infection by gene silencing [J].
Covey, SN ;
AlKaff, NS ;
Langara, A ;
Turner, DS .
NATURE, 1997, 385 (6619) :781-782
[3]   Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells [J].
Elbashir, SM ;
Harborth, J ;
Lendeckel, W ;
Yalcin, A ;
Weber, K ;
Tuschl, T .
NATURE, 2001, 411 (6836) :494-498
[4]   Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans [J].
Fire, A ;
Xu, SQ ;
Montgomery, MK ;
Kostas, SA ;
Driver, SE ;
Mello, CC .
NATURE, 1998, 391 (6669) :806-811
[5]   RNA interference of influenza virus production by directly targeting rnRNA for degradation and indirectly inhibiting all viral RNA transcription [J].
Ge, Q ;
McManus, MT ;
Nguyen, T ;
Shen, CH ;
Sharp, PA ;
Eisen, HN ;
Chen, JZ .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2003, 100 (05) :2718-2723
[6]   An RNA-directed nuclease mediates post-transcriptional gene silencing in Drosophila cells [J].
Hammond, SM ;
Bernstein, E ;
Beach, D ;
Hannon, GJ .
NATURE, 2000, 404 (6775) :293-296
[7]   RNA interference [J].
Hannon, GJ .
NATURE, 2002, 418 (6894) :244-251
[8]  
PETERS K, 1997, ANNU REV BIOCHEM, V66, P523
[9]   A similarity between viral defense and gene silencing in plants [J].
Ratcliff, F ;
Harrison, BD ;
Baulcombe, DC .
SCIENCE, 1997, 276 (5318) :1558-1560
[10]   Gene silencing without DNA: RNA-mediated cross-protection between viruses [J].
Ratcliff, FG ;
MacFarlane, SA ;
Baulcombe, DC .
PLANT CELL, 1999, 11 (07) :1207-1215
12