On-line HPLC-MS-DPPH assay for the analysis of phenolic antioxidant compounds in fruit wine: Antidesma thwaitesianum Muell.

被引：62

作者：

Nuengchamnong, Nitra ^{[2
]}

Ingkaninan, Kornkanok ^{[1
]}

机构：

[1] Naresuan Univ, Dept Pharmaceut Chem & Pharmacognosy, Fac Pharmaceut Sci, Phitsanulok 65000, Thailand

[2] Minist Publ Hlth, Reg Med Sci Ctr Phitsanulok, Dept Med Sci, Phitsanulok 65000, Thailand

来源：

FOOD CHEMISTRY | 2010年 / 118卷 / 01期

关键词：

On-line HPLC-MS-antioxidant assay; Fruit wine; Characterisation; Antidesma thwaitesianum; TANDEM MASS-SPECTROMETRY; ELECTROSPRAY; FLAVONOIDS; CAPACITY;

D O I：

10.1016/j.foodchem.2009.04.069

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

A reversed phase high performance liquid chromatography (RP-HPLC) separation coupled with an electrospray ionisation mass spectrometry detection (ESI-MS) and the 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay was used for the screening of multiple antioxidant compounds in Antidesma thwaitesianum Muell. fruit wine. The active compounds were identified by comparison with authentic standards and published mass spectra. With the help of the multidimensional information of LC-ESI-MS/MS and DPPH assay, the compounds with different chemical structures could be determined in one run successfully. The antioxidant compounds were separated and identified as gallic acid, cyanidin-3-sophoroside, monogalloyl glucoside, delphinidin-3-sambubioside, catechin, caffeic acid, and pelargonidin-3-malonyl glucoside. This result shows that an on-line HPLC-MS-DPPH assay can be a powerful technique for the rapid characterisation of antioxidant compounds in plant extracts. (C) 2009 Elsevier Ltd. All rights reserved.

引用

页码：147 / 152

页数：6

共 16 条

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