Bordetella bronchiseptica aroA mutant as a live vaccine vehicle for heterologous porcine circovirus type 2 major capsid protein expression

被引：11
作者：
Kim, Taejung [1 ]
Toan, Nguyen Tat [1 ]
Seo, Jayoung [1 ]
Jung, Bockgie [1 ]
Lee, Jaeil [1 ]
Lee, Bongjoo [1 ]
机构：
[1] Chonnam Natl Univ, Coll Vet Med, Biotherapy Human Resources Ctr, Kwangju 500757, South Korea
来源：
VETERINARY MICROBIOLOGY | 2009年 / 138卷 / 3-4期
关键词：
Bordetella bronchiseptica; Heterologous antigen expression vehicle; Porcine circovirus type 2; MULTISYSTEMIC WASTING SYNDROME; RESPIRATORY SYNDROME VIRUS; CONVENTIONAL PIGS; ORF2; PROTEIN; PCV2; IMMUNOGENICITY; INFECTION; PCR; CONSTRUCTION; IMMUNIZATION;
D O I：
10.1016/j.vetmic.2009.04.014
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
Porcine circovirus type 2 (PCV2) infections cause important respiratory diseases in the pig industry and are associated with many bacterial, mycoplasmal, and viral complications. In this study, a heterologous PCV2 major capsid protein (MCP) was expressed in the Bordetella bronchiseptica aroA mutant strain (BBS-MCP) and used as a live vaccine vehicle. Mice and pigs were immunized with live BBS-IMCP via the intranasal route. The antibodies against MCP were induced successfully in the serum as determined by ELISA. In the PCV2 challenge experiment, viral DNA was removed successfully from the lymph nodes of pigs vaccinated with live BBS-MCP. Overall, BBS-MCP is believed to be a good candidate for the development of a live-attenuated vaccine against PCV2 infections. (C) 2009 Elsevier B.V. All rights reserved.
引用
收藏
页码：318 / 324
页数：7
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