PROBABLE MOLECULAR MECHANISM OF PANCREATIC CANCER CELLS MIGRATION PROMOTED BY HIGH MOLECULAR WEIGHT HYALURONIC ACID

被引：2

作者：

Jiang, Bing ^{[1
]}

Wu, Qingrong ^{[2
]}

Wan, Yuping ^{[1
]}

Wu, Tingting ^{[3
]}

Liu, Wenjun ^{[4
]}

He, Liuming ^{[5
]}

Pan, Qiwen ^{[6
]}

Tang, Zhaohui ^{[7
]}

机构：

[1] Third Hosp Nanchang, Obstet & Gynecol, Nanchang 330009, Jiangxi, Peoples R China

[2] Fuling Cent Hosp Chongqing City, Dept Gynecol, Chongqing 408000, Peoples R China

[3] First Peoples Hosp Jiujiang City, Dept Gynecol, Jiujiang 332000, Peoples R China

[4] Hangzhou Singclean Med Prod Co Ltd, Mkt Dept, Hangzhou 310000, Zhejiang, Peoples R China

[5] Qinzhou Matern & Child Hlth Care Hosp, Dept Gynecol, Qinzhou City 535099, Peoples R China

[6] Peoples Hosp Hechi, Dept Gynecol, Hechi City 547000, Peoples R China

[7] Cent Hosp Yongzhou, Dept Oncol, 396 Yiyun Rd, Yongzhou City 425000, Peoples R China

来源：

ACTA MEDICA MEDITERRANEA | 2019年 / 35卷 / 04期

基金：

对外科技合作项目（国际科技项目）;

关键词：

High molecular weight; hyaluronic acid; pancreatic cancer; cell migration; molecular mechanism; PROGRESSION;

D O I：

10.19193/0393-6384_2019_4_329

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Objective: To study the molecular mechanism of invasion and metastasis of pancreatic cancer cells promoted by high molecular weight hyaluronic acid. Methods: Real-time PCR and Western blot were used to detect the expression of hyaluronic acid synthase 2 (HAS2) and hyaluro- nidase 1 (HYAL1) that can regulate hyaluronic acid metabolism in pancreatic cancer cell lines and primary pancreatic astrocytes. The effect of HA treatment on expression of hyaluronic acid receptor differentiation cluster antigen 44 (CD44) and hyaluronic acid-medi- ated cell migration receptor (RHAMM) in pancreatic cancer cells was also assessed. Results: Hyaluronic acid in pancreatic cancer tissues mainly existed as high molecular weight hyaluronic acid. Treatment of pancreatic cancer cells with high molecular weight hyaluronic acid promoted migration and inhibited proliferation. Moreover, the knockdown of HYAL1 reduced the ability of high molecular weight hyaluronic acid to promote migration of pancreatic cancer cells. Lastly, CD44 was broken in pancreatic cancer cells after high molecular weight hyaluronic acid treatment. Conclusion: Pancreatic cancer tissue contains a large amount of high molecular weight hyaluronic acid that can promote the migration of pancreatic cancer cells, which may be related to the expression of HYAL1 and the cleavage of CD44.

引用

页码：2099 / 2103

页数：5

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