Alkyl imidazolium chloride ionic liquids (ILs) have been used for numerous biochemical applications. Their hydrophobicity can be tuned by changing the alkyl chain length, and longer-chain ILs can form micelles in aqueous solution. We have investigated the effects of imidazolium chloride ILs on the structure and stability of azurin, which is a very stable Cu2+ redox protein with both alpha-helix and beta-sheet domains. Temperature-dependent infrared (IR) and vibrational circular dichroism spectroscopy can provide secondary-structure-specific information about how the protein is affected, and temperature-jump transient IR measurements can quantify the IL-influenced unfolding dynamics. Using these techniques, we can quantify how azurin is destabilized by 1.0 M ILs in aqueous solution. The shorter, less hydrophobic ILs, 1-butyl-3-methylimidazolium chloride and 1-hexyl-3-methylimidazolium chloride likely interact with the alpha-helix domain and decrease protein melting temperature from 82 degrees C without IL to SS degrees C and disturb the overall tertiary structure, resulting in a looser, more open shape. Thermodynamic analysis indicates that protein destabilization is due to increased unfolding entropy. 1-Octyl-3-methylimidazolium chloride [OMIM]Cl, which forms micelles in solution that may partially solvate the protein, has a more significant destabilizing effect, resulting in a melting temperature of 35 degrees C, larger unfolding entropy, and relaxation kinetics several orders of magnitude faster than with unperturbed azurin. The temperatureindependence of the relaxation time constant suggests that in the presence of [OMIM]Cl, the protein folding potential energy surface has become very smooth.
机构:
Univ Nova Lisboa, Inst Tecnol Quim & Biol, P-2780157 Oeiras, PortugalUniv Nova Lisboa, Inst Tecnol Quim & Biol, P-2780157 Oeiras, Portugal
Alves, Filipa
;
Oliveira, Filipe S.
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机构:
Univ Aveiro, Dept Quim, CICECO, P-3810193 Aveiro, PortugalUniv Nova Lisboa, Inst Tecnol Quim & Biol, P-2780157 Oeiras, Portugal
Oliveira, Filipe S.
;
Schroeder, Bernd
论文数: 0引用数: 0
h-index: 0
机构:
UFP, Escola Super Saude, CIAGEB Fac Ciencias Saude, P-4200150 Oporto, PortugalUniv Nova Lisboa, Inst Tecnol Quim & Biol, P-2780157 Oeiras, Portugal
Schroeder, Bernd
;
Matos, Carla
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h-index: 0
机构:
Univ Nova Lisboa, Inst Tecnol Quim & Biol, P-2780157 Oeiras, PortugalUniv Nova Lisboa, Inst Tecnol Quim & Biol, P-2780157 Oeiras, Portugal
Matos, Carla
;
Marrucho, Isabel M.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Nova Lisboa, Inst Tecnol Quim & Biol, P-2780157 Oeiras, Portugal
Univ Aveiro, Dept Quim, CICECO, P-3810193 Aveiro, PortugalUniv Nova Lisboa, Inst Tecnol Quim & Biol, P-2780157 Oeiras, Portugal
机构:
Inst Max Von Laue Paul Langevin, F-38042 Grenoble, FranceUniv Coll Dublin, Sch Phys, Dublin 4, Ireland
Gonzalez, Miguel A.
;
Fragneto, Giovanna
论文数: 0引用数: 0
h-index: 0
机构:
Inst Max Von Laue Paul Langevin, F-38042 Grenoble, FranceUniv Coll Dublin, Sch Phys, Dublin 4, Ireland
Fragneto, Giovanna
;
Watkins, Erik
论文数: 0引用数: 0
h-index: 0
机构:
Inst Max Von Laue Paul Langevin, F-38042 Grenoble, France
Los Alamos Natl Lab, Lujan Neutron Scattering Ctr, Los Alamos, NM 87545 USAUniv Coll Dublin, Sch Phys, Dublin 4, Ireland
Watkins, Erik
;
Ballone, Pietro
论文数: 0引用数: 0
h-index: 0
机构:
IIT, Ctr Life Nano Sci Sapienza, I-00161 Rome, Italy
Univ Roma La Sapienza, Dept Phys, I-00185 Rome, ItalyUniv Coll Dublin, Sch Phys, Dublin 4, Ireland
机构:
Univ Illinois, Dept Chem, Urbana, IL 61801 USAUniv Illinois, Dept Chem, Urbana, IL 61801 USA
Bhagi-Damodaran, Ambika
;
Reed, Julian H.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Illinois, Dept Biochem, Urbana, IL 61801 USAUniv Illinois, Dept Chem, Urbana, IL 61801 USA
Reed, Julian H.
;
Zhu, Qianhong
论文数: 0引用数: 0
h-index: 0
机构:
Oregon Hlth & Sci Univ, Div Environm & Biomol Syst, Portland, OR 97239 USAUniv Illinois, Dept Chem, Urbana, IL 61801 USA
Zhu, Qianhong
;
Shi, Yelu
论文数: 0引用数: 0
h-index: 0
机构:
Stevens Inst Technol, Dept Chem & Chem Biol, Hoboken, NJ 07030 USAUniv Illinois, Dept Chem, Urbana, IL 61801 USA
Shi, Yelu
;
Hosseinzadeh, Parisa
论文数: 0引用数: 0
h-index: 0
机构:
Univ Illinois, Dept Biochem, Urbana, IL 61801 USAUniv Illinois, Dept Chem, Urbana, IL 61801 USA
Hosseinzadeh, Parisa
;
Sandoval, Braddock A.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Illinois, Dept Chem, Urbana, IL 61801 USAUniv Illinois, Dept Chem, Urbana, IL 61801 USA
Sandoval, Braddock A.
;
Harnden, Kevin A.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Illinois, Dept Chem, Urbana, IL 61801 USAUniv Illinois, Dept Chem, Urbana, IL 61801 USA
Harnden, Kevin A.
;
Wang, Shuyan
论文数: 0引用数: 0
h-index: 0
机构:
Univ Illinois, Dept Chem, Urbana, IL 61801 USAUniv Illinois, Dept Chem, Urbana, IL 61801 USA
Wang, Shuyan
;
Sponholtz, Madeline R.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Illinois, Dept Biochem, Urbana, IL 61801 USAUniv Illinois, Dept Chem, Urbana, IL 61801 USA
Sponholtz, Madeline R.
;
Mirts, Evan N.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Illinois, Ctr Biophys & Quantitat Biol, Urbana, IL 61801 USAUniv Illinois, Dept Chem, Urbana, IL 61801 USA
Mirts, Evan N.
;
Dwaraknath, Sudharsan
论文数: 0引用数: 0
h-index: 0
机构:
Univ Illinois, Dept Chem, Urbana, IL 61801 USAUniv Illinois, Dept Chem, Urbana, IL 61801 USA
Dwaraknath, Sudharsan
;
Zhang, Yong
论文数: 0引用数: 0
h-index: 0
机构:
Stevens Inst Technol, Dept Chem & Chem Biol, Hoboken, NJ 07030 USAUniv Illinois, Dept Chem, Urbana, IL 61801 USA
Zhang, Yong
;
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机构:
Moenne-Loccoz, Pierre
;
Lu, Yi
论文数: 0引用数: 0
h-index: 0
机构:
Univ Illinois, Dept Chem, Urbana, IL 61801 USA
Univ Illinois, Dept Biochem, Urbana, IL 61801 USA
Univ Illinois, Ctr Biophys & Quantitat Biol, Urbana, IL 61801 USAUniv Illinois, Dept Chem, Urbana, IL 61801 USA
机构:
Temple Univ, Inst Computat Mol Sci, Philadelphia, PA 19122 USA
Temple Univ, Dept Chem, Philadelphia, PA 19122 USA
Univ Penn, Res Struct Matter Lab, Philadelphia, PA 19104 USATemple Univ, Inst Computat Mol Sci, Philadelphia, PA 19122 USA
Bhargava, B. L.
;
Yasaka, Yoshiro
论文数: 0引用数: 0
h-index: 0
机构:
Temple Univ, Inst Computat Mol Sci, Philadelphia, PA 19122 USA
Temple Univ, Dept Chem, Philadelphia, PA 19122 USATemple Univ, Inst Computat Mol Sci, Philadelphia, PA 19122 USA
Yasaka, Yoshiro
;
Klein, Michael L.
论文数: 0引用数: 0
h-index: 0
机构:
Temple Univ, Inst Computat Mol Sci, Philadelphia, PA 19122 USA
Temple Univ, Dept Chem, Philadelphia, PA 19122 USATemple Univ, Inst Computat Mol Sci, Philadelphia, PA 19122 USA
机构:
Univ Nova Lisboa, Inst Tecnol Quim & Biol, P-2780157 Oeiras, PortugalUniv Nova Lisboa, Inst Tecnol Quim & Biol, P-2780157 Oeiras, Portugal
Alves, Filipa
;
Oliveira, Filipe S.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Aveiro, Dept Quim, CICECO, P-3810193 Aveiro, PortugalUniv Nova Lisboa, Inst Tecnol Quim & Biol, P-2780157 Oeiras, Portugal
Oliveira, Filipe S.
;
Schroeder, Bernd
论文数: 0引用数: 0
h-index: 0
机构:
UFP, Escola Super Saude, CIAGEB Fac Ciencias Saude, P-4200150 Oporto, PortugalUniv Nova Lisboa, Inst Tecnol Quim & Biol, P-2780157 Oeiras, Portugal
Schroeder, Bernd
;
Matos, Carla
论文数: 0引用数: 0
h-index: 0
机构:
Univ Nova Lisboa, Inst Tecnol Quim & Biol, P-2780157 Oeiras, PortugalUniv Nova Lisboa, Inst Tecnol Quim & Biol, P-2780157 Oeiras, Portugal
Matos, Carla
;
Marrucho, Isabel M.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Nova Lisboa, Inst Tecnol Quim & Biol, P-2780157 Oeiras, Portugal
Univ Aveiro, Dept Quim, CICECO, P-3810193 Aveiro, PortugalUniv Nova Lisboa, Inst Tecnol Quim & Biol, P-2780157 Oeiras, Portugal
机构:
Inst Max Von Laue Paul Langevin, F-38042 Grenoble, FranceUniv Coll Dublin, Sch Phys, Dublin 4, Ireland
Gonzalez, Miguel A.
;
Fragneto, Giovanna
论文数: 0引用数: 0
h-index: 0
机构:
Inst Max Von Laue Paul Langevin, F-38042 Grenoble, FranceUniv Coll Dublin, Sch Phys, Dublin 4, Ireland
Fragneto, Giovanna
;
Watkins, Erik
论文数: 0引用数: 0
h-index: 0
机构:
Inst Max Von Laue Paul Langevin, F-38042 Grenoble, France
Los Alamos Natl Lab, Lujan Neutron Scattering Ctr, Los Alamos, NM 87545 USAUniv Coll Dublin, Sch Phys, Dublin 4, Ireland
Watkins, Erik
;
Ballone, Pietro
论文数: 0引用数: 0
h-index: 0
机构:
IIT, Ctr Life Nano Sci Sapienza, I-00161 Rome, Italy
Univ Roma La Sapienza, Dept Phys, I-00185 Rome, ItalyUniv Coll Dublin, Sch Phys, Dublin 4, Ireland
机构:
Univ Illinois, Dept Chem, Urbana, IL 61801 USAUniv Illinois, Dept Chem, Urbana, IL 61801 USA
Bhagi-Damodaran, Ambika
;
Reed, Julian H.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Illinois, Dept Biochem, Urbana, IL 61801 USAUniv Illinois, Dept Chem, Urbana, IL 61801 USA
Reed, Julian H.
;
Zhu, Qianhong
论文数: 0引用数: 0
h-index: 0
机构:
Oregon Hlth & Sci Univ, Div Environm & Biomol Syst, Portland, OR 97239 USAUniv Illinois, Dept Chem, Urbana, IL 61801 USA
Zhu, Qianhong
;
Shi, Yelu
论文数: 0引用数: 0
h-index: 0
机构:
Stevens Inst Technol, Dept Chem & Chem Biol, Hoboken, NJ 07030 USAUniv Illinois, Dept Chem, Urbana, IL 61801 USA
Shi, Yelu
;
Hosseinzadeh, Parisa
论文数: 0引用数: 0
h-index: 0
机构:
Univ Illinois, Dept Biochem, Urbana, IL 61801 USAUniv Illinois, Dept Chem, Urbana, IL 61801 USA
Hosseinzadeh, Parisa
;
Sandoval, Braddock A.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Illinois, Dept Chem, Urbana, IL 61801 USAUniv Illinois, Dept Chem, Urbana, IL 61801 USA
Sandoval, Braddock A.
;
Harnden, Kevin A.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Illinois, Dept Chem, Urbana, IL 61801 USAUniv Illinois, Dept Chem, Urbana, IL 61801 USA
Harnden, Kevin A.
;
Wang, Shuyan
论文数: 0引用数: 0
h-index: 0
机构:
Univ Illinois, Dept Chem, Urbana, IL 61801 USAUniv Illinois, Dept Chem, Urbana, IL 61801 USA
Wang, Shuyan
;
Sponholtz, Madeline R.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Illinois, Dept Biochem, Urbana, IL 61801 USAUniv Illinois, Dept Chem, Urbana, IL 61801 USA
Sponholtz, Madeline R.
;
Mirts, Evan N.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Illinois, Ctr Biophys & Quantitat Biol, Urbana, IL 61801 USAUniv Illinois, Dept Chem, Urbana, IL 61801 USA
Mirts, Evan N.
;
Dwaraknath, Sudharsan
论文数: 0引用数: 0
h-index: 0
机构:
Univ Illinois, Dept Chem, Urbana, IL 61801 USAUniv Illinois, Dept Chem, Urbana, IL 61801 USA
Dwaraknath, Sudharsan
;
Zhang, Yong
论文数: 0引用数: 0
h-index: 0
机构:
Stevens Inst Technol, Dept Chem & Chem Biol, Hoboken, NJ 07030 USAUniv Illinois, Dept Chem, Urbana, IL 61801 USA
Zhang, Yong
;
论文数: 引用数:
h-index:
机构:
Moenne-Loccoz, Pierre
;
Lu, Yi
论文数: 0引用数: 0
h-index: 0
机构:
Univ Illinois, Dept Chem, Urbana, IL 61801 USA
Univ Illinois, Dept Biochem, Urbana, IL 61801 USA
Univ Illinois, Ctr Biophys & Quantitat Biol, Urbana, IL 61801 USAUniv Illinois, Dept Chem, Urbana, IL 61801 USA
机构:
Temple Univ, Inst Computat Mol Sci, Philadelphia, PA 19122 USA
Temple Univ, Dept Chem, Philadelphia, PA 19122 USA
Univ Penn, Res Struct Matter Lab, Philadelphia, PA 19104 USATemple Univ, Inst Computat Mol Sci, Philadelphia, PA 19122 USA
Bhargava, B. L.
;
Yasaka, Yoshiro
论文数: 0引用数: 0
h-index: 0
机构:
Temple Univ, Inst Computat Mol Sci, Philadelphia, PA 19122 USA
Temple Univ, Dept Chem, Philadelphia, PA 19122 USATemple Univ, Inst Computat Mol Sci, Philadelphia, PA 19122 USA
Yasaka, Yoshiro
;
Klein, Michael L.
论文数: 0引用数: 0
h-index: 0
机构:
Temple Univ, Inst Computat Mol Sci, Philadelphia, PA 19122 USA
Temple Univ, Dept Chem, Philadelphia, PA 19122 USATemple Univ, Inst Computat Mol Sci, Philadelphia, PA 19122 USA