Culturing periprosthetic tissue in BacT/Alert® Virtuo blood culture system leads to improved and faster detection of prosthetic joint infections

被引：18

作者：

Sanabria, Adriana ^{[1
]}

Rokeberg, Merethe E. O. ^{[2
]}

Johannessen, Mona ^{[1
]}

Sollid, Johanna Ericson ^{[1
]}

Simonsen, Gunnar Skov ^{[1
,2
]}

Hanssen, Anne-Merethe ^{[1
]}

机构：

[1] Arctic Univ Norway, Res Grp Host Microbe Interact, Fac Hlth Sci, UiT,Dept Med Biol, Tromso, Norway

[2] Univ Hosp North Norway, Dept Microbiol & Infect Control, Tromso, Norway

来源：

BMC INFECTIOUS DISEASES | 2019年 / 19卷 / 1期

关键词：

BacT/Alert (R) Virtuo blood culture system; Blood culture bottle; Prosthetic joint infection; Periprosthetic tissue specimens; STREPTOCOCCUS-PNEUMONIAE; BACTERIAL CULTURE; DIAGNOSIS; PERFORMANCE; BOTTLES; MEDIA; FLUID; IDENTIFICATION; ARTHROPLASTY; SONICATION;

D O I：

10.1186/s12879-019-4206-x

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

Background: Blood culture bottles (BCBs) provide a semiautomated method for culturing periprosthetic tissue specimens. A study evaluating BCBs for culturing clinical samples other than body fluids is needed before implementation into clinical practice. Our objective was to evaluate use of the BacT/Alert (R) Virtuo blood culture system for culturing periprosthetic tissue specimens. Methods: The study was performed through the analysis of spiked (n = 36) and clinical (n = 158) periprosthetic tissue samples. Clinical samples were analyzed by the BCB method and the results were compared to the conventional microbiological culture-based method for time to detection and microorganisms identified. Results: The BacT/Alert (R) Virtuo blood culture system detected relevant bacteria for prosthetic joint infection in both spiked and clinical samples. The BCB method was found to be as sensitive (79%) as the conventional method (76%) (p = 0.844) during the analyses of clinical samples. The BCB method yielded positive results much faster than the conventional method: 89% against 27% detection within 24 h, respectively. The median detection time was 11.1 h for the BCB method (12 h and 11 h for the aerobic and the anaerobic BCBs, correspondingly). Conclusion: We recommend using the BacT/Alert (R) Virtuo blood culture system for analyzing prosthetic joint tissue, since this detect efficiently and more rapidly a wider range of bacteria than the conventional microbiological method.

引用

页数：8

共 39 条

[11]

Esteban J. P-JC, 2012, INFECT TOTAL JOINT A

[12] Performance of Two Resin-Containing Blood Culture Media in Detection of Bloodstream Infections and in Direct Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry (MALDI-TOF MS) Broth Assays for Isolate Identification: Clinical Comparison of the BacT/Alert Plus and Bactec Plus Systems [J].

Fiori, Barbara ;

D'Inzeo, Tiziana ;

Di Florio, Viviana ;

De Maio, Flavio ;

De Angelis, Giulia ;

Giaquinto, Alessia ;

Campana, Lara ;

Tanzarella, Eloisa ;

Tumbarello, Mario ;

Antonelli, Massimo ;

Sanguinetti, Maurizio ;

Spanu, Teresa .

JOURNAL OF CLINICAL MICROBIOLOGY, 2014, 52 (10) :3558-3567

[13] Blood Culture Flasks for Culturing Synovial Fluid in Prosthetic Joint Infections [J].

Font-Vizcarra, Lluis ;

Garcia, Sebastian ;

Martinez-Pastor, Juan C. ;

Sierra, Josep M. ;

Soriano, Alex .

CLINICAL ORTHOPAEDICS AND RELATED RESEARCH, 2010, 468 (08) :2238-2243

[14] Prospective Comparison of Blood Culture Bottles and Conventional Swabs for Microbial Identification of Suspected Periprosthetic Joint Infection [J].

Geller, Jeffrey A. ;

MacCallum, Katherine P. ;

Murtaugh, Taylor S. ;

Patrick, David A., Jr. ;

Liabaud, Barthelemy ;

Jonna, Venkata K. .

JOURNAL OF ARTHROPLASTY, 2016, 31 (08) :1779-1783

[15] Microbiological diagnosis of prosthetic joint infections: a prospective evaluation of four bacterial culture media in the routine laboratory [J].

Hughes, H. C. ;

Newnham, R. ;

Athanasou, N. ;

Atkins, B. L. ;

Bejon, P. ;

Bowler, I. C. J. W. .

CLINICAL MICROBIOLOGY AND INFECTION, 2011, 17 (10) :1528-1530

[16] Culture with BACTEC Peds Plus/F bottle compared with conventional methods for detection of bacteria in synovial fluid [J].

Hughes, JG ;

Vetter, EA ;

Patel, R ;

Schleck, CD ;

Harmsen, S ;

Turgeant, LT ;

Cockerill, FR .

JOURNAL OF CLINICAL MICROBIOLOGY, 2001, 39 (12) :4468-4471

[17] Multicenter Clinical Evaluation of BacT/Alert Virtuo Blood Culture System [J].

Jacobs, Michael R. ;

Mazzulli, Tony ;

Hazen, Kevin C. ;

Good, Caryn E. ;

Abdelhamed, Ayman M. ;

Lo, Pauline ;

Shum, Bianche ;

Roman, Katharine P. ;

Robinson, Danielle C. .

JOURNAL OF CLINICAL MICROBIOLOGY, 2017, 55 (08) :2413-2421

[18] Projections of primary and revision hip and knee arthroplasty in the United States from 2005 to 2030 [J].

Kurtz, Steven ;

Ong, Kevin ;

Lau, Edmund ;

Mowat, Fionna ;

Halpern, Michael .

JOURNAL OF BONE AND JOINT SURGERY-AMERICAN VOLUME, 2007, 89A (04) :780-785

[19]

Lopez Bernaldo De Quiros Juan Carlos, 1996, Clin Microbiol Infect, V2, P143

[20] Use of an automated blood culture system (BD BACTECTM) for diagnosis of prosthetic joint infections: easy and fast [J].

Minassian, Angela M. ;

Newnham, Robert ;

Kalimeris, Elizabeth ;

Bejon, Philip ;

Atkins, Bridget L. ;

Bowler, Ian C. J. W. .

BMC INFECTIOUS DISEASES, 2014, 14

← 1 2 3 4 →