Optical anisotropy of collagen fibers of rat calcaneal tendons: An approach to spatially resolved supramolecular organization

被引：55

作者：

Vidal, Benedicto de Campos ^{[1
]}

Mello, Maria Luiza S. ^{[1
]}

机构：

[1] Univ Estadual Campinas, Inst Biol, Dept Cell Biol, BR-13083863 Campinas, SP, Brazil

来源：

ACTA HISTOCHEMICA | 2010年 / 112卷 / 01期

基金：

巴西圣保罗研究基金会;

关键词：

Birefringence; Collagen fibers; Crimp; Fast Fourier transform; Tendon; FORM BIREFRINGENCE; TISSUE; ORDER; ORIENTATION; ALIGNMENT; BUNDLES; REPAIR;

D O I：

10.1016/j.acthis.2008.07.005

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Optical anisotropic characteristics investigated by polarization microscopy have been valuable for the study of the oriented organization of collagen fibers in tendons. However, topographic differences in supramolecular organization of collagen fibers along extensive areas in tendons have not yet been described. Here, the statistical. variability of the oriented organization of collagen fibers along extensive areas (10(5)-10(6) mu m(2)) of 7-mu m thick unstained sections of rat calcaneal. tendons were studied by assessing their birefringence with polarization microscopy and image analysis, and the periodic frequency distribution in their birefringent images with the fast Fourier transform (FFT). Various levels of birefringence intensity were determined principally by image analysis procedures, and periodicity in the birefringent images was revealed by FFT line profile and spectrum image patterns. Present results support the idea of a helical distribution for collagen bundles along the tendon long axis, and of a statistical architecture for the rat calcaneal tendons in terms of variability in the oriented distribution of their collagen fibers. (C) 2008 Elsevier GmbH. All rights reserved.

引用

页码：53 / 61

页数：9

共 30 条

[11] Form birefringence of oxidized porous silicon [J].

Golovan, L. A. ;

Ivanov, D. A. ;

Melnikov, V. A. ;

Timoshenko, V. Yu. ;

Zheltikov, A. M. ;

Kashkarov, P. K. ;

Petrov, G. I. ;

Yakovlev, V. V. .

APPLIED PHYSICS LETTERS, 2006, 88 (24)

[12] Analysis of surface second-harmonic generation by orientational distribution function in a chiral polymer film [J].

Ha, NY ;

Han, SH ;

Jeon, DW ;

Jung, CS ;

Park, B ;

Takezoe, H ;

Wu, JW .

PHYSICAL REVIEW E, 2005, 72 (03)

[13] The Fourier analysis of biological transients [J].

Harris, CM .

JOURNAL OF NEUROSCIENCE METHODS, 1998, 83 (01) :15-34

[14]

HASSAN SA, 2002, PHYS REV E, V65, P1

[15] Birefringence induced by irregular structure in photonic crystal fiber [J].

Hwang, IK ;

Lee, YJ ;

Lee, YH .

OPTICS EXPRESS, 2003, 11 (22) :2799-2806

[16]

MELLO MLS, 1979, GERONTOLOGY, V25, P2, DOI 10.1159/000212314

[17] All-optical control of microfluidic components using form birefringence [J].

Neale, SL ;

Macdonald, MP ;

Dholakia, K ;

Krauss, TF .

NATURE MATERIALS, 2005, 4 (07) :530-533

[18] Chiroptical effects in the second harmonic signal of collagens I and IV [J].

Pena, AM ;

Boulesteix, T ;

Dartigalongue, T ;

Schanne-Klein, MC .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2005, 127 (29) :10314-10322

[19] 2ND HARMONIC-GENERATION AND ORIENTATIONAL ORDER IN CONNECTIVE-TISSUE - A MOSAIC MODEL FOR FIBRIL ORIENTATIONAL ORDERING IN RAT-TAIL TENDON [J].

ROTH, S ;

FREUND, I .

JOURNAL OF APPLIED CRYSTALLOGRAPHY, 1982, 15 (FEB) :72-78

[20] Form-birefringent space-variant inhomogeneous medium element for shaping point-spread functions [J].

Tsai, CH ;

Levy, U ;

Pang, L ;

Fainman, Y .

APPLIED OPTICS, 2006, 45 (08) :1777-1784

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