Enzyme-Assisted Release of Antioxidant Peptides from Porphyra dioica Conchocelis

被引：7

作者：

Pimentel, Filipa B. ^{[1
]}

Machado, Marlene ^{[1
]}

Cermeno, Maria ^{[2
]}

Kleekayai, Thanyaporn ^{[2
]}

Machado, Susana ^{[1
]}

Rego, Andreia M. ^{[3
]}

Abreu, Maria H. ^{[3
]}

Alves, Rita C. ^{[1
]}

Oliveira, Maria Beatriz P. P. ^{[1
]}

FitzGerald, Richard J. ^{[2
]}

机构：

[1] Univ Porto, Dept Chem Sci, Fac Pharm, LAQV,REQUIMTE, P-4050313 Porto, Portugal

[2] Univ Limerick, Dept Biol Sci, Prot & Peptides Res Grp, Limerick V94 T9PX, Ireland

[3] ALGAplus Prod & Comercializacao Algas & Seus Deri, P-3830196 Ilhavo, Portugal

来源：

ANTIOXIDANTS | 2021年 / 10卷 / 02期

关键词：

Porphyra dioica conchocelis; amino acids; enzyme-assisted release; reactive oxygen species; antioxidant activity;

D O I：

10.3390/antiox10020249

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The conchocelis life cycle stage of P. dioica represents an unexplored source of bioactive compounds. The aim of this study was to generate and characterise, for the first time, hydrolysates of conchocelis using a specific combination of proteases (Prolyve (R) and Flavourzyme (R)). Hydrolysate molecular mass distribution and free amino acid contents were assessed, and the antioxidant activity was determined using a range of in vitro assays. The protein content and the total amino acid profiles of conchocelis were also studied. Conchocelis contained similar to 25% of protein (dry weight basis) and had a complete profile of essential amino acids. Direct sequential enzymatic treatment modified the profile of the generated compounds, increasing the amount of low molecular weight peptides (<1 kDa). There was a significant improvement in the antioxidant activity of the hydrolysates compared with the control (up to 2.5-fold), indicating their potential as a novel source of antioxidant ingredients.

引用

页码：1 / 13

页数：13

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