Effect of 1-Methylcyclopropene on the Expression of Genes for Ascorbate Metabolism in Postharvest Cauliflower

被引：1

作者：

Ma, Gang ^{[1
,2
]}

Zhang, Lancui ^{[1
]}

Kato, Masaya ^{[1
]}

Yamawaki, Kazuki ^{[1
]}

Asai, Tatsuo ^{[1
]}

Nishikawa, Fumie ^{[3
]}

Ikoma, Yoshinori ^{[4
]}

Matsumoto, Hikaru ^{[4
]}

机构：

[1] Shizuoka Univ, Fac Agr, Shizuoka 4228529, Japan

[2] Shizuoka Univ, Gifu Univ, United Grad Sch Agr Sci, Gifu 5011193, Japan

[3] Natl Inst Fruit Tree Sci, Kuchinotsu Citrus Res Stn, Kuchinotsu, Minamishimabara 8592501, Japan

[4] Natl Inst Fruit Tree Sci, Okitsu Citrus Res Stn, Shizuoka 4240292, Japan

来源：

JOURNAL OF THE JAPANESE SOCIETY FOR HORTICULTURAL SCIENCE | 2011年 / 80卷 / 04期

关键词：

ascorbate; cauliflower; gene expression; 1-methylcyclopropene (1-MCP); senescence; VITAMIN-C CONTENT; ETHYLENE BIOSYNTHESIS; GLUTATHIONE CYCLE; FRUIT-DEVELOPMENT; MOLECULAR-BIOLOGY; OXIDATIVE STRESS; VAR; BOTRYTIS; ACC SYNTHASE; BRASSICA; BROCCOLI;

D O I：

10.2503/jjshs1.80.512

中图分类号：

S6 [园艺];

学科分类号：

0902 ;

摘要：

The effects of 1-methylcyclopropene (1-MCP) on ascorbate (AsA) metabolism were studied and the possible molecular mechanisms were discussed for two cultivars of cauliflower (Brassica oleracea, L. var. botrytis), 'Violet Queen' and 'Snow Crown'. Ethylene production was suppressed in 'Violet Queen', while it was increased in 'Snow Crown' by 1-MCP treatment. Meanwhile, the changing patterns of AsA metabolism after harvest were different between the two cauliflower cultivars. In 'Violet Queen', AsA content decreased in the control after harvest, and the loss of AsA was delayed by 1-MCP treatment. In 'Snow Crown', AsA content remained almost constant and was not affected by 1-MCP treatment. In 'Violet Queen', the gene expression of BO-APX1, BO-APX2, and BO-sAPX was down-regulated, while the gene expression of BO-DHAR and BO-GLDH was up-regulated by 1-MCP treatment. The regulation of these genes contributed to the suppression of AsA reduction in 'Violet Queen' treated by 1-MCP. In 'Snow Crown', simultaneous down-regulation of BO-APX1, BO-APX2, and BO-sAPX, which were responsible for AsA breakdown, and BO-MDAR1, BO-MDAR2, BO-DHAR, and BO-GLDH, which were responsible for AsA regeneration and biosynthesis, might lead to maintain the AsA level constant with 1-MCP treatment.

引用

页码：512 / 520

页数：9

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