APC/CCdh1 Enables Removal of Shugoshin-2 from the Arms of Bivalent Chromosomes by Moderating Cyclin-Dependent Kinase Activity

被引：6

作者：

Rattani, Ahmed ^{[1
,7
,8
]}

Mejia, Randy Ballesteros ^{[2
,3
]}

Roberts, Katherine ^{[1
]}

Roig, Maurici B. ^{[1
]}

Godwin, Jonathan ^{[1
]}

Hopkins, Michael ^{[1
]}

Eguren, Manuel ^{[4
]}

Sanchez-Pulido, Luis ^{[5
]}

Okaz, Elwy ^{[1
]}

Ogushi, Sugako ^{[1
]}

Wolna, Magda ^{[1
]}

Metson, Jean ^{[1
]}

Pendas, Alberto M. ^{[6
]}

Malumbres, Marcos ^{[4
]}

Novak, Bela ^{[1
]}

Herbert, Mary ^{[2
,3
]}

Nasmyth, Kim ^{[1
]}

机构：

[1] Univ Oxford, Dept Biochem, South Parks Rd, Oxford OX1 3QU, England

[2] Newcastle Fertil Ctr, Ctr Life, Times Sq, Newcastle Upon Tyne NE1 4EP, Tyne & Wear, England

[3] Newcastle Univ, Inst Med Genet, Wellcome Trust Ctr Mitochondrial Res, Newcastle Upon Tyne NE4 5PL, Tyne & Wear, England

[4] Spanish Natl Canc Res Ctr CNIO, Cell Div & Canc Grp, Madrid 28029, Spain

[5] Univ Edinburgh, Inst Genet & Mol Med, MRC Human Genet Unit, Edinburgh EH4 2XU, Midlothian, Scotland

[6] Univ Salamanca, CSIC, Inst Biol Mol & Celular Canc Salamanca, Salamanca 37007, Spain

[7] Harvard Med Sch, Mt Auburn Hosp, Cambridge, MA 02138 USA

[8] Harvard Med Sch, Dept Syst Biol, Boston, MA 02115 USA

来源：

CURRENT BIOLOGY | 2017年 / 27卷 / 10期

基金：

英国生物技术与生命科学研究理事会; 英国工程与自然科学研究理事会; 英国惠康基金; 英国医学研究理事会; 欧盟地平线“2020”;

关键词：

ANAPHASE-PROMOTING COMPLEX; SPINDLE ASSEMBLY CHECKPOINT; SISTER-CHROMATID SEPARATION; KINETOCHORE-MICROTUBULE ATTACHMENTS; MOUSE OOCYTES; MEIOSIS-I; PREVENTS NONDISJUNCTION; REQUIRES PROTEOLYSIS; APC(CDH1) ACTIVITY; MAMMALIAN OOCYTES;

D O I：

10.1016/j.cub.2017.04.023

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In mammalian females, germ cells remain arrested as primordial follicles. Resumption of meiosis is heralded by germinal vesicle breakdown, condensation of chromosomes, and their eventual alignment on metaphase plates. At the first meiotic division, anaphase-promoting complex/cyclosome associated with Cdc20 (APC/C-Cdc20) activates separase and thereby destroys cohesion along chromosome arms. Because cohesion around centromeres is protected by shugoshin-2, sister chromatids remain attached through centromeric/pericentromeric cohesin. We show here that, by promoting proteolysis of cyclins and Cdc25B at the germinal vesicle (GV) stage, APC/C associated with the Cdh1 protein (APC/C-Cdh1) delays the increase in Cdk1 activity, leading to germinal vesicle breakdown (GVBD). More surprisingly, by moderating the rate at which Cdk1 is activated following GVBD, APC/C-Cdh1 creates conditions necessary for the removal of shugoshin-2 from chromosome arms by the Aurora B/C kinase, an event crucial for the efficient resolution of chiasmata.

引用

页码：1462 / +

页数：20