Differential Expression of Circular RNAs in Glioblastoma Multiforme and Its Correlation with Prognosis

被引：99

作者：

Zhu, Junle ^{[1
]}

Ye, Jingliang ^{[1
,2
]}

Zhang, Lei ^{[1
]}

Xia, Lili ^{[3
]}

Hu, Hongkang ^{[1
]}

Jiang, Heng ^{[4
]}

Wan, Zhiping ^{[1
]}

Sheng, Fei ^{[5
]}

Ma, Yan ^{[5
]}

Li, Wen ^{[5
]}

Qian, Jun ^{[1
]}

Luo, Chun ^{[1
]}

机构：

[1] Second Mil Med Univ, Changzheng Hosp, Dept Neurosurg, 415 Fengyang Rd, Shanghai 200003, Peoples R China

[2] Chinese Peoples Liberat Army, Hosp 98, Dept Neurosurg, Huzhou 313000, Peoples R China

[3] Second Mil Med Univ, Changzheng Hosp, Dept Emergency, 415 Fengyang Rd, Shanghai 200003, Peoples R China

[4] Second Mil Med Univ, Changzheng Hosp, Dept Spinal Surg, 415 Fengyang Rd, Shanghai 200003, Peoples R China

[5] Second Mil Med Univ, Changzheng Hosp, Dept Reprod, Med Ctr, 415 Fengyang Rd, Shanghai 200003, Peoples R China

来源：

TRANSLATIONAL ONCOLOGY | 2017年 / 10卷 / 02期

基金：

中国国家自然科学基金;

关键词：

ABUNDANT; BRAIN; IDENTIFICATION; PROGRESSION; BIOMARKER; GROWTH; GENE;

D O I：

10.1016/j.tranon.2016.12.006

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

OBJECTIVE: The present study aimed to explore the expression profiles of circular RNAs (circRNAs) in glioblastoma multiforme (GBM) in an attempt to identify potential core genes in the pathogenesis of this tumor. METHODS: Differentially expressed circRNAs were screened between tumor tissues from five GBM patients and five normal brain samples using Illumina Hiseq. Bioinformatics analysis was used to analyze their potential function. CircBRAF was further detected in different WHO grades glioma tissues and normal brain tissues. Kaplan-Meier curves and multivariate Cox's analysis were used to analyze the association between circBRAF expression level and prognosis of glioma patients. RESULTS: A total of 1411 differentially expressed circRNAs were identified in GBM patients including 206 upregulated circRNAs and 1205 downregulated circRNAs. Differential expression of circRNAs was closely associated with the biological process and molecular function. The downregulated circRNAs were mainly associated with ErbB and Neurotrophin signaling pathways. Moreover, the expression level of circBRAF in normal brain tissues was significantly higher than that in glioma tissues (P < .001). CircBRAF was significantly lower in glioma patients with high pathological grade (WHO III & IV) than those with low grade (WHO I&II) (P < .001). Cox analysis revealed that high circBRAF expression was an independent biomarker for predicting good progression-free survival and overall survival in glioma patients (HR = 0.413, 95% CI 0.201-0.849; HR = 0.299, 95% CI 0.135-0.661; respectively). CONCLUSION: The present study identified a profile of dysregulated circRNAs in GBM. Bioinformatics analysis showed that dysregulated circRNAs might be associated with tumorigenesis and development of GBM. In addition, circBRAF could severe as a biomarker for predicting pathological grade and prognosis in glioma patients.

引用

页码：271 / 279

页数：9

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