Dullard promotes degradation and dephosphorylation of BMP receptors and is required for neural induction

被引:69
作者
Satow, Reiko
Kurisaki, Akira
Chan, Te-chuan
Hamazaki, Tatsuo S.
Asashima, Makoto
机构
[1] Univ Tokyo, Grad Sch Sci, Dept Biol Sci, Bunkyo Ku, Tokyo 1138654, Japan
[2] Univ Tokyo, Grad Sch Arts & Sci, Dept Life Sci Biol, Meguro Ku, Tokyo 1538902, Japan
[3] Int Med Ctr Japan, Dept Tissue Regenerat, Inst Res, Shinjyuku Ku, Tokyo 1628655, Japan
[4] Japan Sci & Technol JST Agcy, Oran Regenerat Project, Meguro Ku, Tokyo 1538902, Japan
[5] Univ Tokushima, Inst Enzyme Res, Dept Mol Cytol, Tokushima 7708503, Japan
[6] Natl Inst Adv Ind Sci & Technol, Organ Dev Res Lab, Tsukuba, Ibaraki 3058562, Japan
基金
日本学术振兴会;
关键词
D O I
10.1016/j.devcel.2006.10.001
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Bone morphogenetic proteins (BMPs) regulate multiple biological processes, including cellular proliferation, adhesion, differentiation, and early development. In Xenopus development, inhibition of the BMP pathway is essential for neural induction. Here, we report that dullard, a gene involved in neural development, functions as a negative regulator of BMP signaling. We show that Dullard promotes the ubiquitin-mediated proteosomal degradation of BMP receptors (BMPRs). Dullard preferentially complexes with the BMP type 11 receptor (BMPRII) and partially colocalizes with the caveolin-1-positive compartment, suggesting that Dullard promotes BMPR degradation via the lipid raft-caveolar pathway. Dullard also associates with BMP type I receptors and represses the BMP-dependent phosphorylation of the BMP type I receptor. The phosphatase activity of Dullard is essential for the degradation of BMP receptors and neural induction in Xenopus. Together, these observations suggest that Dullard is an essential inhibitor of BMP receptor activation during Xenopus neuralization.
引用
收藏
页码:763 / 774
页数:12
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