Desulfurization of dibenzothiophene by lyophilized cells of Pseudomonas delafieldii R-8 in the presence of dodecane

被引：77

作者：

Luo, MF ^{[1
]}

Xing, JM ^{[1
]}

Gou, ZX ^{[1
]}

Li, S ^{[1
]}

Liu, HZ ^{[1
]}

Chen, JY ^{[1
]}

机构：

[1] Chinese Acad Sci, Young Scientist Lab Separat Sci & Engn, State Key Lab Biochem Engn, Inst Proc Engn, Beijing 100080, Peoples R China

来源：

BIOCHEMICAL ENGINEERING JOURNAL | 2003年 / 13卷 / 01期

关键词：

biodesulfurization; dibenzothiophene; dodecane; Pseudomonas delafieldii;

D O I：

10.1016/S1369-703X(02)00078-5

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Several parameters that influence the dibenzothiophene (DBT) desulfurization by lyophilized cells of Pseudomonas delafieldii R-8 were studied in the presence of dodecane. The aqueous media tested with pH range in 4.6-8.5 made no obvious difference on the desulfurization activity. The rate and extent of desulfurization were strongly dependent on the volume ratio of oil-to-water, DBT concentration and the cell concentration. The specific desulfurization rate of DBT and 4,6-dimethyl DBT (4,6-DMDBT) could reach 11.4 and 9.4 mmol sulfur kg(-1) dry cells (DCW) h(-1), respectively. The desulfurization pattern of DBT was represented by the Michaelis-Menten equation. The kinetic parameters, the limiting maximal velocity (V-max) and Michaelis constant (K-m), for desulfurization of DBT were calculated. (C) 2002 Elsevier Science B.V. All rights reserved.

引用

页码：1 / 6

页数：6

共 23 条

[1]

Chang JH, 2000, BIOTECHNOL LETT, V22, P193

[2] CHARACTERIZATION OF THE DESULFURIZATION GENES FROM RHODOCOCCUS SP STRAIN IGTS8 [J].