Analysis of Cellular Activity and Induction of Inflammation in Response to Short-Term Exposure to Cobalt and Chromium Ions in Mature Human Osteoblasts

被引：31

作者：

Jonitz-Heincke, Anika ^{[1
]}

Sellin, Marie-Luise ^{[1
]}

Seyfarth, Anika ^{[1
]}

Peters, Kirsten ^{[2
]}

Mueller-Hilke, Brigitte ^{[3
]}

Fiedler, Tomas ^{[4
]}

Bader, Rainer ^{[1
]}

Klinder, Annett ^{[1
]}

机构：

[1] Rostock Univ, Med Ctr, Dept Orthoped, Biomech & Implant Technol Res Lab, Doberaner Str 142, D-18057 Rostock, Germany

[2] Rostock Univ, Med Ctr, Dept Cell Biol, Schillingallee 69, D-18057 Rostock, Germany

[3] Rostock Univ, Med Ctr, Inst Immunol, Schillingallee 70, D-18057 Rostock, Germany

[4] Rostock Univ, Med Ctr, Inst Med Microbiol Virol & Hyg, Schillingallee 70, D-18057 Rostock, Germany

来源：

MATERIALS | 2019年 / 12卷 / 17期

关键词：

osteoblasts; corrosion; ions; particles; osteolysis; inflammation; METAL-IONS; BIOLOGICAL RESPONSE; HIP-REPLACEMENT; WEAR DEBRIS; TOXICITY; CORROSION; IMPLANTS;

D O I：

10.3390/ma12172771

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

In aseptic loosening of endoprosthetic implants, metal particles, as well as their corrosion products, have been shown to elicit a biological response. Due to different metal alloy components, the response may vary depending on the nature of the released corrosion product. Our study aimed to compare the biological effects of different ions released from metal alloys. In order to mimic the corrosion products, different metal salts (CoCl2, NiCl2 and CrCl3 x 6H(2)O) were dissolved and allowed to equilibrate. Human osteoblasts were incubated with concentrations of 10 mu M to 500 mu M metal salt solutions under cell culture conditions, whereas untreated cells served as negative controls. Cells exposed to CoCr28Mo6 particles served as positive controls. The cell activity and expression of osteogenic differentiation and pro-osteolytic mediators were determined. Osteoblastic activity revealed concentration- and material-dependent influences. Collagen 1 synthesis was reduced after treatment with Co(2+) and Ni(2+). Additionally, exposure to these ions (500 mu M) resulted in significantly reduced OPG protein synthesis, whereas RANKL as well as IL-6 and IL-8 secretion were increased. TLR4 mRNA was significantly induced by Co(2+) and CoCr28Mo6 particles. The results demonstrate the pro-osteolytic capacity of metal ions in osteoblasts. Compared to CoCr28Mo6 particles, the results indicated that metal ions intervene much earlier in inflammatory processes.

引用

页数：17

共 43 条

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