Cell cycle-dependent protein expression of mammalian homologs of yeast DNA double-strand break repair genes Rad51 and Rad52

被引:104
作者
Chen, FQ
Nastasi, A
Shen, ZY
Brenneman, M
Crissman, H
Chen, DJ
机构
[1] LOS ALAMOS NATL LAB,DIV LIFE SCI,MS M888,LOS ALAMOS,NM 87545
[2] UNIV NEW MEXICO,SCH MED,DEPT CELL BIOL,ALBUQUERQUE,NM 87131
来源
MUTATION RESEARCH-DNA REPAIR | 1997年 / 384卷 / 03期
关键词
DNA double strand break repair; Rad51; Rad52; cell cycle; radiation;
D O I
10.1016/S0921-8777(97)00020-7
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Recently, human and rodent homologs of yeast repair genes Rad51 and Rad52 have been identified and proposed to play roles in DNA double-strand break (DSB) repair. In this study, cell cycle-dependent expression of human and rodent RAD51 and RAD52 proteins was monitored using two approaches. First, flow cytometric measurements of DNA content and immunofluorescence were used to determine the phase specific levels of RAD51 and RAD52 protein expression in irradiated and control populations. The expression of both proteins was lowest in G(0)/G(1), increased in S and reached a maximum in G(2)/M. No difference was found in the whole-cell level of RAD51 or RAD52 protein expression between gamma-irradiated and control cell populations. Second, cell cycle-dependent protein expression was confirmed by Western analysis of populations synchronized in G(0), G(1) and G(2) phases. Analysis of V3, a hamster equivalent of SCID, indicates that the protein level increases of RAD51 and RAD52 from G(0) to G(1)/S/G(2) do not require DNA-PK. (C) 1997 Elsevier Science B.V.
引用
收藏
页码:205 / 211
页数:7
相关论文
共 49 条
[1]  
ABE H, 1994, ANTICANCER RES, V14, P1807
[2]   GENETIC-EVIDENCE FOR DIFFERENT RAD52-DEPENDENT INTRACHROMOSOMAL RECOMBINATION PATHWAYS IN SACCHAROMYCES-CEREVISIAE [J].
AGUILERA, A .
CURRENT GENETICS, 1995, 27 (04) :298-305
[3]  
AJIMURA M, 1993, GENETICS, V133, P51
[4]   ROLES FOR THE YEAST RAD18 AND RAD52 DNA-REPAIR GENES IN UV MUTAGENESIS [J].
ARMSTRONG, JD ;
CHADEE, DN ;
KUNZ, BA .
MUTATION RESEARCH-DNA REPAIR, 1994, 315 (03) :281-293
[5]  
ASHLEY T, 1995, CHROMOSOMA, V104, P19, DOI 10.1007/BF00352222
[6]   A Rad52 homolog is required for RAD51-independent mitotic recombination in Saccharomyces cerevisiae [J].
Bai, Y ;
Symington, LS .
GENES & DEVELOPMENT, 1996, 10 (16) :2025-2037
[7]   NUCLEOTIDE-SEQUENCE AND TRANSCRIPTIONAL REGULATION OF THE YEAST RECOMBINATIONAL REPAIR GENE RAD51 [J].
BASILE, G ;
AKER, M ;
MORTIMER, RK .
MOLECULAR AND CELLULAR BIOLOGY, 1992, 12 (07) :3235-3246
[8]   PURIFICATION AND CHARACTERIZATION OF THE HUMAN RAD51 PROTEIN, AN ANALOG OF ESCHERICHIA-COLI RECA [J].
BENSON, FE ;
STASIAK, A ;
WEST, SC .
EMBO JOURNAL, 1994, 13 (23) :5764-5771
[9]   RECA HOMOLOGS DMC1 AND RAD51 INTERACT TO FORM MULTIPLE NUCLEAR-COMPLEXES PRIOR TO MEIOTIC CHROMOSOME SYNAPSIS [J].
BISHOP, DK .
CELL, 1994, 79 (06) :1081-1092
[10]   Disruption of DNA-PK in Ku80 mutant xrs-6 and the implications in DNA double-strand break repair [J].
Chen, FQ ;
Peterson, SR ;
Story, MD ;
Chen, DJ .
MUTATION RESEARCH-DNA REPAIR, 1996, 362 (01) :9-19