Evaluation of pH gradient formation of carrier ampholytes with synthesized isoelectric point markers in capillary isoelectric focusing

被引：12

作者：

Kobayashi, H ^{[1
]}

Aoki, M ^{[1
]}

Suzuki, M ^{[1
]}

Yanagisawa, A ^{[1
]}

Arai, E ^{[1
]}

机构：

[1] BECKMAN INSTRUMENTS LTD,SHIBUYA KU,TOKYO 151,JAPAN

来源：

JOURNAL OF CHROMATOGRAPHY A | 1997年 / 772卷 / 1-2期

关键词：

pH gradients; Isoelectric point markers; ampholytes; peptides; proteins;

D O I：

10.1016/S0021-9673(97)00101-5

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Carrier ampholytes, peptides from protamine digested by alpha-chymotrypsin and two peptides, Gly-Gly-Gly and Gly-Gly-His, were labeled with dansyl chloride isoelectric point (pI) markers for evaluating pH formation in isoelectric focusing. These Dns-labeled ampholytes were partially purified by reversed-phase chromatography and/or preparative isoelectric focusing by rotatory cell and their pi values were estimated by comigration with protein-pi markers. These labeled markers were used to evaluate the resolution and the range of pH forming ability of carrier ampholytes.

引用

页码：137 / 144

页数：8

共 11 条

[1]

GRAY WR, 1972, METHOD ENZYMOL, V25, P128

[2] ADAPTATION OF THE EQUIPMENT FOR HIGH-PERFORMANCE ELECTROPHORESIS TO ISOELECTRIC-FOCUSING [J].