17β-Estradiol protects against the effects of a high fat diet on cardiac glucose, lipid and nitric oxide metabolism in rats

被引：13

作者：

Zafirovic, Sonja ^{[1
]}

Obradovic, Milan ^{[1
]}

Sudar-Milovanovic, Emina ^{[1
]}

Jovanovic, Aleksandra ^{[1
]}

Stanimirovic, Julijana ^{[1
]}

Stewart, Alan J. ^{[2
]}

Pitt, Samantha J. ^{[2
]}

Isenovic, Esma R. ^{[1
]}

机构：

[1] Univ Belgrade, Inst Nucl Sci Vinca, Lab Radiobiol & Mol Genet, Mike Petrovica Alasa 12-14, Belgrade 11000, Serbia

[2] Univ St Andrews, Sch Med, St Andrews KY16 9TF, Fife, Scotland

来源：

MOLECULAR AND CELLULAR ENDOCRINOLOGY | 2017年 / 446卷 / 0C期

关键词：

Estradiol; Obesity; Myocardial metabolism; Cardiovascular diseases; Inducible nitric oxide synthase; INDUCED INSULIN-RESISTANCE; FRUCTOSE-RICH DIET; OBESE ZUCKER RATS; ESTROGEN-RECEPTOR; IN-VIVO; ACID TRANSPORTERS; SKELETAL-MUSCLE; NA+/K+-ATPASE; L-ARGININE; ER-ALPHA;

D O I：

10.1016/j.mce.2017.02.001

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

The aim of this study was to investigate the in vivo effects of 17 beta-estradiol (E-2) on myocardial metabolism and inducible nitric oxide synthase (iNOS) expression/activity in obese rats. Male Wistar rats were fed with a normal or a high fat (HF) diet (42% fat) for 10 weeks. Half of the HF fed rats were treated with a single dose of E-2 while the other half were placebo-treated. 24 h after treatment animals were sacrificed. E-2 reduced cardiac free fatty acid (FFA) (p < 0.05), L-arginine (p < 0.01), iNOS mRNA (p < 0.01), and protein (p < 0.05) levels and translocation of the FFA transporter (CD36) (p < 0.01) to the plasma membrane (PM) in HF fed rats. In contrast, Akt phosphorylation at Thr308 (p < 0.05) and translocation of the glucose transporter GLUT4 (p < 0.05) to the PM increased after E-2 treatment in HF rats. Our results indicate that E-2 acts via the PI3K/Akt signalling pathway to partially protect myocardial metabolism by attenuating the detrimental effects of increased iNOS expression/activity in HF fed rats. (C) 2017 Elsevier B.V. All rights reserved.

引用

页码：12 / 20

页数：9

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