Comprehensive lipidomic, metabolomic and proteomic profiling reveals the role of immune system in vitiligo

被引：16
作者：
Liang, L. [1 ]
Li, Y. [2 ]
Tian, X. [3 ]
Zhou, J. [2 ,4 ]
Zhong, L. [5 ]
机构：
[1] Peking Univ, Sch Basic Med Sci, Hlth Sci Ctr, Dept Biophys, Beijing, Peoples R China
[2] Peking Univ, Sch Basic Med Sci, Hlth Sci Ctr, Dept Pathol, Beijing, Peoples R China
[3] Jilin Peoples Hosp, Jilin, Jilin, Peoples R China
[4] Beihang Univ, Beijing Adv Innovat Ctr Big Data Based Precis Med, Beijing 100191, Peoples R China
[5] Peking Univ, Ctr Med & Hlth Anal, Hlth Sci Ctr, Beijing, Peoples R China
来源：
CLINICAL AND EXPERIMENTAL DERMATOLOGY | 2019年 / 44卷 / 07期
基金：
中国国家自然科学基金;
关键词：
PLATELET-ACTIVATING-FACTOR; GENOME-WIDE ASSOCIATION; CLINICAL-PATTERN; EXPRESSION; INDUCTION; DISEASES; ONSET; CELLS; LOCI;
D O I：
10.1111/ced.13961
中图分类号：
R75 [皮肤病学与性病学];
学科分类号：
100206 ;
摘要：
Background Vitiligo is a common depigmentation disorder resulting from destruction of melanocytes, and has both genetic and environmental influences. Although genomic analyses have been performed to investigate the pathogenesis of vitiligo, the lipidomics, metabolomics and proteomics of serum have not been reported, and the role of small molecules and serum proteins in vitiligo remains unknown. Aim To study the metabolite and protein profiles in patients with vitiligo and healthy controls (HCs). Methods Plasma samples from 60 participants (29 patients with vitiligo and 31 HCs) were analysed. Untargeted lipidomics, metabolomics and isobaric tags for relative and absolute quantification-based proteomics were performed using high performance liquid chromatography-tandem mass spectrometry. In addition, to validate differentially expressed metabolites in patients with vitiligo, plasma enzyme-linked immunosorbent assay was performed. Results We identified differential expression of several metabolites and proteins involved in the immune system. Among these metabolites and proteins, lysophosphatidylcholine, platelet-activating factor, sn-glycerol-3-phosphocholine, succinic acid, CXCL4 and CXCL7 were significantly elevated in the plasma of patients with vitiligo, while aspartate was downregulated. Conclusion Our study has characterized several serum metabolites and proteins that could be potential candidate biomarkers in vitiligo, and provides a comprehensive insight into the role of immune system and aspartate metabolism in vitiligo.
引用
收藏
页码：E216 / E223
页数：8
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