Development and in vitro assay of oxidative stress modifying formulations for wound healing promotion

被引：7

作者：

Atrux-Tallau, Nicolas ^{[1
]}

Callejon, Sylvie ^{[2
]}

Migdal, Camille ^{[2
]}

Padois, Karine ^{[1
]}

Bertholle, Valerie ^{[1
]}

Denis, Alain ^{[3
]}

Chavagnac-Bonneville, Marlene

Haftek, Marek ^{[2
,3
]}

Falson, Francoise ^{[1
]}

Pirot, Fabrice ^{[4
]}

机构：

[1] Univ Lyon, Physiol & Pathol Funct Cutaneous Barrier EA4169, Lab R&D Ind Pharmaceut Technol, F-69373 Lyon 08, France

[2] Univ Lyon, Physiol & Pathol Funct Cutaneous Barrier EA4169, Lab Dermatol Res, E Herriot Hosp, F-69437 Lyon 03, France

[3] Lab Dermatolog Bioderma, F-69447 Lyon 03, France

[4] E Herriot Hosp, Pharm Dept Drugs Fabricat & Control, F-69437 Lyon 03, France

来源：

EUROPEAN JOURNAL OF DERMATOLOGY | 2011年 / 21卷

关键词：

skin wound healing; hydrogen peroxide; chitosan; alginate; keratinocyte; ENDOTHELIAL GROWTH-FACTOR; EMULSIFICATION-INTERNAL GELATION; KERATINOCYTE CELL-LINE; HYDROGEN-PEROXIDE; SUBSTRATE-DEPENDENCE; BIPHASIC REGULATION; GLUCOSE-OXIDASE; ALGINATE BEADS; NADPH OXIDASE; EXPRESSION;

D O I：

10.1684/ejd.2011.1270

中图分类号：

R75 [皮肤病学与性病学];

学科分类号：

100206 ;

摘要：

Often presented as metabolism byproducts, reactive oxygen species are linked to detrimental effects such as chronic wound, mutagenesis, cancer and skin ageing. However, recent in vitro and in vivo observations suggest that ROS, and mainly hydrogen peroxide, interfere with cell signaling acting like second messenger and inducing adaptive responses. This is particularly observed in skin wound healing where cells are exposed to H2O2 following injury. In this study, we developed and characterized an innovative formulation producing H2O2 at low concentrations, in order to mimic physiological inflammation phase. Then, this pro-oxidative formulation (CAM-GOx) was assayed in vitro on keratinocytes cell culture, compared to the blank formulation (CAM) and the anti-oxidative formulation (CAM-CAT) to assess whether oxidative stress was implied or not in cellular responses.

引用

页码：52 / 62

页数：11

共 52 条

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