High-Resolution 3D NIR-II Photoacoustic Imaging of Cerebral and Tumor Vasculatures Using Conjugated Polymer Nanoparticles as Contrast Agent

被引：95

作者：

Guo, Bing ^{[1
]}

Chen, Jingqin ^{[2
,3
]}

Chen, Ningbo ^{[2
]}

Middha, Eshu ^{[1
]}

Xu, Shidang ^{[1
]}

Pan, Yutong ^{[1
]}

Wu, Min ^{[1
]}

Li, Ke ^{[2
]}

Liu, Chengbo ^{[2
]}

Liu, Bin ^{[1
]}

机构：

[1] Natl Univ Singapore, Dept Chem & Biomol Engn, 4 Engn Dr 4, Singapore 117585, Singapore

[2] Chinese Acad Sci, CAS Key Lab Hlth Informat, Res Lab Biomed Opt & Mol Imaging, Inst Biomed & Hlth Engn,Shenzhen Inst Adv Technol, Shenzhen 518055, Peoples R China

[3] Univ Chinese Acad Sci, Shenzhen Coll Adv Technol, Shenzhen 518055, Peoples R China

来源：

ADVANCED MATERIALS | 2019年 / 31卷 / 25期

基金：

中国国家自然科学基金;

关键词：

3D biological imaging; angiography; conjugated polymer nanoparticles; microfluidics; photoacoustic imaging; MICROSCOPY; DOTS; PORPHYRIN; NM;

D O I：

10.1002/adma.201808355

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Exogenous contrast-agent-assisted NIR-II optical-resolution photoacoustic microscopy imaging (ORPAMI) holds promise to decipher wide-field 3D biological structures with deep penetration, large signal-to-background ratio (SBR), and high maximum imaging depth to depth resolution ratio. Herein, NIR-II conjugated polymer nanoparticle (CP NP) assisted ORPAMI is reported for pinpointing cerebral and tumor vasculatures. The CP NPs exhibit a large extinction coefficient of 48.1 L g(-1) at the absorption maximum of 1161 nm, with an ultrahigh PA sensitivity up to 2 mu g mL(-1). 3D ORPAMI of wide-field mice ear allows clear visualization of regular vasculatures with a resolution of 19.2 mu m and an SBR of 29.3 dB at the maximal imaging depth of 539 mu m. The margin of ear tumor composed of torsional dense vessels among surrounding normal regular vessels can be clearly delineated via 3D angiography. In addition, 3D whole-cortex cerebral vasculatures with large imaging area (48 mm(2)), good resolution (25.4 mu m), and high SBR (22.3 dB) at a depth up to 1001 mu m are clearly resolved through the intact skull. These results are superior to the recently reported 3D NIR-II fluorescence confocal vascular imaging, which opens up new opportunities for NIR-II CP-NP-assisted ORPAMI in various biomedical applications.

引用

页数：9