Fibre-optic genosensor for specific determination of femtomolar DNA oligomers

被引：51

作者：

Kleinjung, F ^{[1
]}

Bier, FF ^{[1
]}

Warsinke, A ^{[1
]}

Scheller, FW ^{[1
]}

机构：

[1] UNIV POTSDAM, INST BIOCHEM & MOL PHYSIOL, MAX DELBRUCK CTR MOL MED, D-13125 BERLIN, GERMANY

来源：

ANALYTICA CHIMICA ACTA | 1997年 / 350卷 / 1-2期

关键词：

DNA-probe; biosensors; fiber optic fluorescence;

D O I：

10.1016/S0003-2670(97)00192-X

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The binding of DNA oligonucleotides to immobilized DNA-targets using a fibre optic fluorescence sensor is demonstrated. 13mer oligonucleotides were attached to the core of a multimode fibre. The complementary sequence was detected by use of a fluorescent double strand specific DNA ligand (YOYO and PicoGreen). The evanescent field was employed to distinguish between bound and not bound species. The template DNA-oligomer was immobilized either by direct coupling to the activated sensor surface or using the avidin-biotin bridge. Single base mismatches in the target sequence were detected; and a detection limit of the sensor of 30 fM (3.2 attomoles) was found for the matching target.

引用

页码：51 / 58

页数：8

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